摘要
目的 :初步探讨人羊膜诱导小鼠胚胎干细胞向表皮样细胞定向分化的机理。方法 :小鼠胚胎干细胞与人羊膜在双层 6孔培养板中共培养 4 - 5d ,对照组未加羊膜 ,观察其形态学分化。分别用β1整合素、角蛋白 19/15和套膜蛋白免疫组化检测胚胎干细胞向表皮样细胞的分化。结果 :共培养 4 - 5d后 ,小鼠胚胎干细胞分化为表皮细胞样的单层细胞 ,细胞排列紧密 ,呈多边形 ,免疫组织化学染色显示 ,大部分细胞呈现 β1整合素阳性 ,少数细胞呈角蛋白 19和角蛋白 15阳性 ,未见套膜蛋白阳性细胞 ,对照组大部分细胞死亡 ,存活细胞形态各异 ,未见β1整合素、角蛋白 19/15和套膜蛋白免疫组化染色阳性细胞。结论
AIM: To investigate the mechanism by which human amnion induced mouse embryonic stem (ES) cells to differentiate into epidermal like cells. METHODS: ES-BALB/c cells were cocultured with human amnion in transwells for 4-5 days, and those cultured alone without amnion were taken as control group. The morphological differentiation were observed. The committed differentiation of ES cells into epidermal like cells were detected by integrin-β_1, CK19, CK15 and involucrin immunohistochemistry, respectively. RESULTS: After 4-5 days of coculture, ES cells differentiated into single layer of epidermal like cells, fitted tightly, with polyhedral in shape. The immunohistochemical staining results showed that, most of the cells were integin-β_1 positive, only a few cells were CK19 and CK15 positively stained. Most of the cells in control group died, the survived ones were different in morphological shapes, and no integrin-β_1, CK19 and CK15 positive cells were found. CONCLUSION: Soluble substances secreted by human amnion may play an important role in inducing the differentiation of mouse ES cells into epidermal like cells. [
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第7期1163-1166,共4页
Chinese Journal of Pathophysiology
基金
国家重点基础研究课题 (9. 73)资助项目(G19990 5 4 30 1- 2 )
关键词
胚胎干细胞
羊膜
细胞分化
表皮细胞
Embryonic stem cells
Amnion
Cell differentiation
Epidermal cells
