摘要
目的 研究参麦注射液 (SMI)对过氧化氢 (H2 O2 )或血管紧张素Ⅱ (AngⅡ )所致心肌细胞损害的保护作用及其机制。方法 根据加入细胞培养液中的H2 O2 、AngⅡ、SMI、维生素C(VitC)浓度不同分为 15组。检查指标有心肌细胞培养液中乳酸脱氢酶 (LDH )、丙二醛 (MDA)、过氧化物歧化酶 (SOD)及心肌细胞存活力(CMV)、Na+ K+ ATP酶、Ca2 + ATP酶、心肌细胞凋亡率 (CMAR)、一氧化氮 (NO)、NO合成酶 (NOS)、内皮型NOS(eNOS)的表达。结果 H2 O2 或AngⅡ均可降低CMV、CMAR、SOD、Na+ K+ ATP酶、Ca2 + ATP酶、NOS活性、NO含量、eNOS表达 ,增高CMAR、LDH及MDA。SMI可有效减轻H2 O2 或AngⅡ所致上述指标变化 ,SMI 10ml/L作用强于 5ml/L及VitC 5 0mg/L。 结论 SMI对H2 O2
Objective To investigate the protective effects and mechanism of shenmai injection(SMI) on cardiomyocytes injured by hydrogen peroxide(H 2O 2) or angiotension Ⅱ(AngⅡ). Methods According to the different concentrations of H 2O 2, AngⅡ,SMI,Vitamin C(VitC) added into the cardiomyocytes culture media(CCM), the cultures were divided into 15 groups. Lactate dehydrogenase(LDH ),malondialdehyde(MDA),superoxide dismutase (SOD) of CCM and cardiomyocytes viability(CMV), Na +-K +-ATPase,Ca 2+-ATPase?cardiomyocytes apoptosis rate(CMAR) ,nitrogen oxide(NO), nitrogen oxide synthesis enzyme(NOS),eNOS expression of cardiomyocytes were detected respectively .Results H 2O 2 or AngⅡ could decrease CMV, SOD, Na +-K +-ATPase,Ca 2+-ATPase,NOS,NO,eNOS expression and increase CMAR,LDH and MDA.SMI could lessen the changes of the items mentioned above ,which were caused by H 2O 2 or AngⅡ.The effects of SMI 10 ml/L were stronger than those of SMI 5 ml/L or VitC 50 mg/L.Conclusion SMI has a significant protective effect on cardiomyocyte injured by H 2O 2 or AngⅡ.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第7期551-553,共3页
Journal of Applied Clinical Pediatrics
基金
山东省科技厅计划课题 (1 999BB1DBA1 )
