摘要
本文报道了反相液相色谱(RP-HPLC)和离子对反相液相色谱甘氨型(G)和牛磺型(T)结合胆汁酸的选择性变化规律,首次发现在给定冲洗剂浓度下,G、T对结合型胆汁酸的选择性因子或结合型选择性为常数。本文同时还在反相液相色谱保留值基本方程的基础上讨论了G、T对选择性变化规律的理论依据。
In this paper, the selectivity factors or the conjugation selectivity between glycine andtaurine conjugated bile acids in reversed-phase high-performance liquid chromatography (RP-HPLC) and ion-pair RP-HPLC (IP-RPHPLC) have been found to be constant at the fixedconcentration of the mobile phase. The conjugation selectivity has been emplained based on theretention equation log k′=a+c C_B in RP-HPLC.
出处
《分析测试学报》
CAS
CSCD
1993年第3期7-10,共4页
Journal of Instrumental Analysis
关键词
结合型
胆汁酸
高效液相色谱
反相
RP-HPLC
IP-RPHPLC
Conjugation selectivity
Conjugated bile acids
