摘要
目的 :研究蛙皮素 (BN)能否拮抗大鼠干扰素 (IFN)性发热反应及其可能机制的探讨。方法 :建立大鼠IFN α性发热模型 ,观察侧脑室注射BN对大鼠IFN α性发热反应及下丘脑、脑腹中隔区 (VSA)精氨酸加压素 (AVP)含量的影响。结果 :①侧脑室注射IFN α(每只 2、5、8× 10 4U)引起剂量依赖性体温升高 ,同时测得VSA中AVP含量明显增加 (P <0 .0 5 ) ,下丘脑AVP含量无明显变化 (P >0 .0 5 )。②侧脑室注射BN(每只 0 .1、0 .5 μg) ,引起剂量依赖性体温降低 (P <0 .0 1) ,同时测得VSA中AVP含量增加 (P <0 .0 5 ) ,下丘脑AVP含量无明显变化 (P >0 .0 5 )。③侧脑室注射IFN α(每只 5× 10 4U) 30min后 ,侧脑室注射BN(每只 0 .5 μg) ,BN能逆转大鼠发热反应 (P <0 .0 5 ) ,并于 15 0min时 ,大鼠体温恢复到对照组水平 ,同时测得VSA中AVP含量与对照组无显著性差异 (P >0 .0 5 )。结论 :内生性AVP可能参与了IFN α性发热反应的调节 ;
Aim: To determine whether bombesin prevents IFN-α-induced fever and it's possible mechanism. Methods:Effects of BN on changes in body temperature and arginine vasopressin(AVP) content in the ventral septal area(VSA) and hypothalamus were measured in the rats following intracerebroventricular(ICV) injection of IFN-α. Results: ①IFN-α produced a dose-dependent rise in colonic temperature simultaneously with increase in AVP content in the VSA in the rats.② BN produced a dose-dependent hypothermia and significantly elevated AVP content in the VSA in rats. ③BN injected intracerebroventricularly at 30 min after IFN-α prevented the increase in colonic temperature which recovered to the control level as well as AVP content in the VSA in rats at 150 min. Conclusion: AVP in the VSA may play a role in IFN-α-induced fever. AVP in the VSA may play a partial role in the BN antipyretic action and hypothermic action.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2004年第3期300-303,共4页
Chinese Journal of Applied Physiology
基金
辽宁省自然科学基金资助项目 ( 972 2 2 9)
