摘要
cDNA阵列、基因芯片技术、iAFLP(introduced amplified fragment lengthpolymorphism)、SAGE(serial analysis of gene expression)技术、cDNA-AFLP、DDRT-PCR(differntialdisplay reverse-transcription PCR)、RC4D(RFLP-coupled differential display)以及抑制消减杂交技术(suppression subtractive hybridization,SSH)都是能高效鉴别两个不同细胞群差异表达基因的方法。现对各种方法的原理进行简要介绍,并对其优缺点进行对比,并结合作者实验室的研究工作,列举了cDNA阵列和抑制消减杂交技术在生殖生物学领域中的应用情况。
cDNA array, gene chip, iAFLP (introduced AFLP), SAGE (serial analysis of gene expression), cDNA-AFLP, DDRT-PCR, RC4D (RFLP-coupled differential display) and SSH (suppression subtractive hybridization) are techniques being used extensively to identify differentially regulated genes in organisms. In this review, these display systems are evaluated and compared. The general principles on which the different techniques are based and which determine their potential and their limitations are described. Some examples are given about the utilization of these methods.
出处
《细胞生物学杂志》
CSCD
北大核心
2004年第4期339-343,共5页
Chinese Journal of Cell Biology
