摘要
揭示云南白族 136例无关男性个体Y染色体小卫星DYF15 5S1基因座多态性。用已建立的Amp FLP和MVR PCR方法分析DYF15 5S1基因座长度多态性、5′端多态性、3′端多态性。DYF15 5S1基因座具有高度多态性 ,基因多样性 (h)达 0 9996。研究表明将Amp FLP和荧光MVR PCR结合起来更能充分揭示DYF15 5S1基因座多态性 ,可为遗传学、人类学及法医学的研究提供有效的工具和基础资料。
This study is to reveal the polymorphism of DYF155S1 locus, Y-specific minisatellite, among 136 cases unrelated male in Bai population of Yunnan Province. The analytical methods include Amp-FLP(Amplified fragment length polymorphism) and MVR-PCR(minisatellite variant repeat by PCR)with type 1-specific primer at 5′ end and type 4-specific primer at 3′ end. It is found that DYF155S1 locus possesses a high polymorphism and its gene diversity (h) reaches 0.9996. These results indicated that Amp-FLP in combination with MVR-PCR may reveal fully the polymorphism of DYF155S1 locus and provide a useful tools and more data for the field of human genetics, anthropology and forensic medicine.
出处
《人类学学报》
CSCD
北大核心
2004年第3期239-247,共9页
Acta Anthropologica Sinica
基金
广东省自然科学基金资助项目 (97 12 8)
中山医科大学"2 11工程"重点学科建设课题基金资助项目 (4 2 0 90 0 8)
