摘要
从多种成分的组合配方中研制成一种增菌效果显著的MA_(12)促细菌生长因子,在禽多杀性巴氏杆菌的培养基中,加入0.05—0.15％MA_(12)可替代3—5％的血(清)浓渡,二者繁殖菌数相似。以此制备的禽霍乱荚膜亚单位苗对家禽具有良好的免疫原性。MA_(12)是一种含植物生长素、微量元素等物质的增菌剂,可高压灭菌,使用方便,是一种廉价的血清代用品。本研究系国内首次报道。
One kind of growth-stimulating factor, MA12 which has an obvious bacterium-increasing effect had been prepared. Adding 0. 05-0. 15%MA12 to the culture growing Pasteurella multacida of Fowl Cholera has similar effect on increasing bacterial amount to compare with adding 3-5% animal serum in the culture. The cell capsule antigen prepared with serum-free culture has good immunity for poultry. MA12 is mainly consisted of plant growth-regulating substrate and microelements. It is easy to be gathered with low cost and convinient use, also can be sterilized by autoclave. This is the first report on using scrum-free medium to produce capsule subunit vaccine in China.
关键词
增菌剂
巴氏杆菌
细胞荚膜抗原
Serum-free medium
Bacterial synergist
Pasteurella multocida
Fowl cholera
Cell capsule antigen
