摘要
本实验采用过碘酸钠法用辣根过氧化物酶标记纯化的I型鸭肝炎病毒单克隆抗体,建立了检测DHV(鸭病毒性肝炎)的Dot-ELISA方法。该方法简便易行,特异性好,与鸭瘟病毒、小鹅瘟病毒、鸡传染性法氏囊病毒、鸡新城疫病毒等无交叉反应,重复性高,对病毒尿囊液的最低检出范围为116,而对肝脏病料的最低检出范围为1160。该方法可用于临床发病鸭的检测。
A Dot-ELISA was developed for detecting duck hepatitis virus with horseradish peroxidase-labelled DHV Mc-antibody. The method was simple, special, repetitive and sensitive for detection of duck hepatitis virus.The detection limits of the DHV was 1:16 and 1:160.It can be used for detection of field infected ducks.
出处
《山东家禽》
2004年第9期6-8,共3页
Shandong Poultry
