摘要
目的研究原发性肝癌患者 TAE 术前后外周血中微转移变化情况,并评价 AFP mRNA 的预后诊断价值。方法应用巢式 RT-PCR 方法,检测32例肝癌患者 TAE 术前后外周血中 AFP mRNA 的表达,并对其预后进行随访观察。结果 32例 TAE 患者术前 AFP mRNA 阳性21例,阳性率为65.6％;术后 AFP mRNA 阳性23例,阳性率为71.9％。其中21例术前阳性者依然全部阳性,未发现有转阴性;11例阴性患者中有2例转为阳性。随访观察发现 AFP mRNA 阳性患者肝内、肝外转移发生率和 AFP mRNA 阴性患者差异显著(p<0.01)。结论 TAE 可能促使原发性肝癌发生血道播散。检测肝癌患者外周血中的 AFP mRNA,是一种灵敏预后诊断方法。
To study the influence of TAE for patients with hepatocellular carcinoma on disseminating tumor cells in peripheral blood by demonstrating hepatocyte-associated gene AFP mRNA and to evaluate the value of AFPmRNA for prognosis of HCC.Methods AFP mRNA in peripheral blood in 32 cases with HCC which were performed by TAE were detected by means of nested reverse transcriptase-polymerase chain reaction between pre-and post-operation.and patients have been followed up to assess the prognoses.Result Human AFP mRNA was detected in 21 of 32 cases of HCC (65. 6%) before TAE was performed.Following operation was done,21 positive cases was still positive and no one changed into negative;but 2 negative ones were becoming positive.ALL patients were followed up about 14-22 months.there are significant differences between positive and negative acses in intrhepatic and extrahepaic metastasis.Conclusion TAE may actuate primary tumor cells to spread into blood.AFP mRNA detected by nested reverse transcriptase-ploymerase chain re- action is a very sensitive method to dope out prognosis of patients with HHC.
出处
《现代消化及介入诊疗》
2000年第3期20-23,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
