摘要
以褐藻裙带菜(Undaria pinnatifida)为实验材料,采用蔗糖密度梯度超速离心的方法,去污剂SDS为增溶剂(SDS:Chl=20:1,4℃增溶20 min),蔗糖密度梯度为60%、50%、40%、30%、20%、15%和10%,分离制备光系统Ⅰ(PSⅠ)复合物。结果表明, 40% 蔗糖层带所含色素蛋白复合物是PSⅠ复合物。利用红藻作参照对比,光谱结果表明从裙带菜中得到的PSⅠ复合物没有730 nm的荧光峰。分析认为这是所有褐藻包括裙带菜PSⅠ复合物的荧光特异性。
A technique for separating the pigment-protein complexes from brown algae Undariapinnatifida had some improvement in this work. Pigment-protein complexes were isolated by cen-trifugation on discontinuous sucrose density gradient of the SDS-treated thylakoid membranes. Thebest separation was obtained with detergent SDS (SDS:Chl=20:1, solubilized 20 min at 4℃), andsucrose density gradients (10%,15%,20%,30%,40%,50%,60% W/W). The 40% sucrose layer repre-sented photosystemⅠ (PSⅠ) complex. In contrast with red algae, the results suggested that PSⅠcomplex from Undaria pinnatifida had no symbol of 730 nm fluorescence peak.
出处
《植物学通报》
CAS
CSCD
北大核心
2004年第4期444-448,共5页
Chinese Bulletin of Botany
基金
国家自然基金重点项目(39890390)。
