摘要
为研究中药治疗骨质疏松症的作用机理。采用体外分离培养大鼠破骨细胞并分为空白组、固邦组和骨康组 ,分别添加相应的药物血清共同培养 ,然后进行指标检测。结果骨康组HE染色可见破骨细胞胞浆浓缩、核固缩深染 ,有的细胞出现核碎裂 ,TRAP阳性细胞数明显减少 ,象牙骨片上的吸收陷窝数量及面积减少 ,流式细胞仪监测时在G1期前出现凋亡峰 ,共聚焦显微镜观察可见细胞凋亡征象。检测结果接近固邦组 ,但与空白组具有显著性差异。表明中药骨康是通过抑制体外培养的破骨细胞活性并能诱导破骨细胞凋亡 ,而起到抑制骨吸收的作用的。
In order to study the action mechanism of traditional Chinese drugs for treating osteoporosis, isolated and cultivated rat osteoclasts were divided into three groups: the blank (B), 'GUBANG' (GB) and 'GUKANG' (GK), which were cultivated in the mediums with corresponding medicine serums, respectively; then the indices of all groups were detected. The results showed that in GK group, the HE staining showed osteoclast plasma concentrated, karyopyknosis deeply stained and karyorrexis appearing in some cells; the positive cells stained by TRAP and the counting and area of absorption lacuna in the ivory sclerites were significantly decreased; the flow cytometer detected the whithering-peak appearance before GI stage; focusing microscopy showed the osteoclasts whithering sign, all of which were similar to those in GB group but obviously different from those in B group, suggesting that TCD GK could inhibit the bone absorption through inhibiting in-vitro-cultured osteoclasts activity and inducing osteoclasts whithering.
出处
《中医正骨》
2004年第6期3-5,共3页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
广东省中医药管理局资助项目 (编号 :10 10 80 )
