摘要
目的:直接进样高效液相色谱法测定人血浆中的头孢布烯的含量。方法:选用浸透限制固定相,紫外检测器,使头孢布烯在流动相pH 7.0条件下与内标及血浆中的蛋白质得到良好的分离。结果:制作了头孢布烯的标准曲线,测定方法的变异系数在4.1%～7.3%,回收率近100%。结论:浸透限制固定相色谱法测定血浆中的头孢布烯,省去了前处理,快速简单,更适用于临床治疗药物监测。
Objective:To establish a new method to assay ceftibuten in plasma by high performance liquid chromatography (HPLC).Methods:The ceftibuten were well separated from the internal standard and plasma proteins at pH 7.0 by HPLC with restricted access stationary phases and UV de- tector. The standard curve of ceftibuten was established. Results: The recovery rates were 97.9 %-101.6% , and the coefficients of variation were 4.1 %- 7. 3 % . Conclusion: This method is easy and ac-curate to determine ceftibuten in plasma by HPLC with restricted access stationary phases. It may be applicable to quantify ceftibuten in clinic.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2004年第6期531-533,共3页
Chinese Journal of New Drugs
