摘要
背景:一些与视网膜色素上皮(retinalpigmentepithelium,RPE)细胞凋亡相关的疾病均与炎症刺激有关。目的:探讨用吲哚美辛诱导体外培养的人胚胎视网膜色素上皮细胞的凋亡。设计:随机对照、单盲法的前瞻性研究。地点和对象:实验在中南大学湘雅二院实验中心进行,胎儿标本来源于中南大学湘雅二院妇产科。干预:加入终浓度分别为100,200,400,600μmol/L的吲哚美辛作用24h;600μmol/L作用6,12,24后用透射电镜定性观察凋亡细胞,并用吖啶橙荧光染色进行定量和定性分析。主要观察指标:凋亡RPE细胞形态和数量的变化。结果:经200,400,600μmol/L吲哚美辛作用24h后RPE细胞凋亡数为(2.7000±1.3375),(5.1000±1.3703),(6.8000±1.6865),与对照组(0.2000±0.4216)相比,差异有显著性意义(t值分别为4.624,9.062,12.206,P<0.01),随着吲哚美辛浓度的增加,RPE细胞凋亡数逐渐增多;600μmol/L吲哚美辛作用12,24h后RPE细胞凋亡数为(4.4000±0.8433),(6.4000±1.2649),与对照组(0.2000±0.4216)相比,差异有显著性意义(t值分别为8.617,13.274,P<0.01)。结论:吲哚美辛能诱导体外培养的人胚胎RPE细胞的凋亡,并且RPE细胞的凋亡呈现出随浓度增加和时间延长凋亡细胞数明显增多。
BACKGROUND:Some diseases related to the apoptosis of retinal pigment epithelial(RPE) cells are all related to inflammatory stimulation. OBJECTIVE:To investigate the apoptosis of in vitro cultivated human fetal RPE cells induced by indomethacin(IN). DESIGN: A randomized controlled and single blind prospective study was conducted. SETTING and PARTICIPANTS:The experiment was completed in the Laboratory of the Second Affiliated Hospital of Xiangya Medical College,Central South University.The fetal specimens were obtained from the Department of Obstetrics and Gynecology of this hospital. INTERVENTIONS:The apoptotic cells were qualitatively observed with a transmission electron microscope(TEM) after being affected by 100,200,400,600 μmol/L(final concentration) IN for 24 hours and 600 μmol/L IN for 6, 12, 24 hours. Quantitative and qualitative analysis was performed through acridine orange(AO) fluorescent staining. MAIN OUTCOME MEASURES:Changes of figures and quantities of apoptotic RPE cells. RESULTS:The numbers of apoptotic RPE cells affected by 200, 400, 600 μmol/L IN for 24 hours were (2.700 0±1.337 5),(5.100 0±1.370 3),(6.800 0±1.686 5) with significant difference,compared with control group(0.200 0±0.421 6)(t=4.624, 9.062, 12.206, P< 0.01). With the increase of IN concentration, the numbers of apoptotic RPE cells increased gradually. The numbers of apoptotic RPE cells affected by 600 μmol/L IN for 12 and 24 hours were (4.400 0±0.843 3),(6.400 0±1.264 9) with significant difference,compared with control group(0.200 0±0.421 6)(t=8.617, 13.274,P< 0.01). CONCLUSION: IN can induce the apoptosis of in vitro cultivated human fetal RPE cells and the numbers of apoptotic cells increase obviously when the concentration and the time increase.
出处
《中国临床康复》
CSCD
2004年第20期4126-4127,i006,共3页
Chinese Journal of Clinical Rehabilitation
