摘要
目的 研究白细胞介素 8(IL 8)、中性粒细胞黏附功能与急性肺损伤 (AL I)的关系。方法 18只雄性新西兰家兔随机分为两组 :对照组 8只 ,AL I组 10只。建立内毒素 AL I模型。测定两组动物血气、血常规、中性粒细胞 CD11b表达强度、血清 IL 8和丙二醛浓度 ,并进行肺组织病理学观察。结果 AL I组动物注射内毒素后出现血压降低、心率减慢、p H进行性下降。给药后 3h外周血中性粒细胞下降明显 ,6 h有不同程度的上升 ;中性粒细胞表面 CD11b表达强度与血清 IL 8、丙二醛浓度呈进行性上升 ,均较对照组差异显著(P均 <0 .0 5 )。模型动物肺组织炎性粒细胞浸润明显 ,弥漫性肺泡隔增厚 ,灶性出血和纤维蛋白渗出。给药后相关分析显示 :6 h时 CD11b与 IL 8呈明显相关 (决定系数 r2 =0 .813,回归方程 Y=2 6 .72 9X ) ;肺组织病理改变严重程度评分与 CD11b表达强度亦呈明显相关 (决定系数 r2 =0 .771,回归方程 Y=0 .0 110 2 X +5 .2 92 )。结论 内毒素可以诱导产生大量 IL 8,使中性粒细胞表面 CD11b的表达被快速激活并上调 ,并与 IL 8和肺组织病理改变评分之间呈高度相关。 IL 8和 CD11b均是 AL
Objective To explore the relationship of interleukin8 (IL8), adherence function of neutrophil (PMN) and acute lung injury (ALI). Methods Eighteen male New Zealand rabbits were divided into two groups randomly: the control group ( n =8) and the ALI group ( n =10). The model of ALI was replicated by using intravenous lipopolysaccaride(LPS) in the rabbits of group ALI. Blood pressure, heart rate, blood gas analysis, hemogram, CD11b expression intensity on the surface of PMN,concentration of serum IL8 and malondialdehyde(MDA) were measured at each time point. Specimens for pathology were obtained at the end of experiment. Results In group ALI, blood pressure, heart rate and pH declined obviously . PMN count was lowered obviously at 3 hours and increased at 6 hours to some extent. CD11b expression intensity on the surface of PMN, the concentration of serum IL8 and MDA were increased progressively (all P <0 05). The main changes in the microscopic examination were inflammatory granulocyte infiltration, disseminated thickening of alveolar septa and focal hemorrhages.There were significant correlations between CD11b and IL8( r 2=0 813, Y =26 729 X ), and between CD11b and the grades of pathological changes at 6 hours after intravenous LPS( r 2=0 771, Y =0 011 02 X +5 292). Conclusion LPS could induce the release of large amount of IL8, and it activates the expression of CD11b on the surface of PMN, which shows high degree of correlation with IL8 and the degree of pathological changes in the lung. Therefore, both of them could serve as sensitive indexes for the diagnosis of ALI.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第7期403-408,共6页
Chinese Critical Care Medicine
