摘要
frlftflf背景:水飞蓟宾(silybin)具有抗自由基活性、抗脂质过氧化作用和抗脂氧酶作用、极低的毒性,但由于其人体生物利用度低,故影响其疗效。水飞蓟宾-磷脂酰胆碱复合物(Silybin-phosphatidylcholinecompound,SPC)具有较好的生物利用度,并且SPC中所含磷脂酰胆碱也具有抗自由基活性、抗脂质过氧化作用,理论上可以认为SPC具有更好的药理作用。目的:了解水飞蓟宾-磷脂酰胆碱复合物(SPC)对大鼠脑缺血再灌注损伤的保护作用。设计:完全随机设计,对照实验研究。地点和材料:所有研究工作均在新乡医学院药物研究室进行。水飞蓟宾(Silybin,含量98%),辽宁盘锦第三制药厂提供;水飞蓟宾-磷脂酰胆碱复合物(SPC,含量>95%),本室合成;丙二醛(MDA)试剂盒,南京建成生物工程研究所提供。仪器:SHIMADZU2550紫外-可见光分光光度计,武汉科学仪器厂DL-6低温离心机。实验动物有新乡医学院动物中心提供。干预:各组受试大鼠给与供试样品灌胃;灌胃容积为0.5mL/100g。将SPC和Silybin分别悬浮于5g/L羧甲基纤维素钠(CMC)溶液中;①伪手术组和②单纯脑缺血-再灌注模型组(单纯模型组):2次/d给与5g/LCMC灌胃。③SPC-1组:每日2次给与SPC5mg/100g(5g/LCMC悬浮液)灌胃。④SPC-2组:2次/d次给与SPC10mg/100g(5g/LCMC悬浮液)灌胃。⑤Silybin组:每?
BACKGROUND:Silybin has the reactions of anti-free radicals,anti-lipoid perox idation,and anti-lipoid oxidase with extremely low toxicity.However,the effecti veness is affected by its low biological utilization in organisms. Silybin-phos phatidylcholine compound(SPC) has preferably biological utility,and moreover,the phosphatidylcholine in SPC also has anti-free radical and anti-lipoid peroxid ation reaction,and therefore,theoretically,SPC has better pharmacological reacti ons. OBJECTIVE:To understand the protective reaction of SPC in cerebral ischemic re perfusion injury in rats. DESIGN:A complete randomized controlled study. SETTING and PARTICIPANTS:Study was conducted in the Department of Pharmacy of Xinxiang Medical College.Silybin(98%) was obtained from Panjin No.3 Pharmaceut ical Factory of Liaoning Province.SPC(>95%) was synthesized by our department.M DA reagent box was obtained from Nanjing Jiancheng Bioengineering Institute.Inst ruments:SHIMADZU 2550 model Ultraviolet-visible light spectrophotometer,DL-6 m odel low-temperature centrifuge(Wuhan Scientific Instrument Factory).Experiment al animals were obtained from the Experimental Animal Center of Xinxiang Medical College. INTERVENTIONS:Sample medications were given through stomach-perfusion to the rats in each group.The volume of stomach-perfusion was 0.5 mL/100 g.SPC and Sil ybin were suspended separately in 5 g/L of CMC solution①Sham-operation group a nd ②Cerebral ischemic-reperfusion group(model group):stomach-perfusion were c onducted twice a day with 5 g/L of CMC.③SPC-1 group: stomach-perfusion were c onducted twice a day with 5 mg/100 g SPC(5 g/L of CMC suspension).④SPC-2 group :stomach-perfusion were conducted twice a day with 10 mg/100 g SPC(5 g/L of CMC suspension);and⑤Silybin group:stomach-perfusion were conducted twice a day wi th 10 mg/100 g of Silybin(5 g/L of CMC suspension).At 2 hours after the sixth st omach-perfusion(on the third day), surgeries were operated in the rats of each group.100 g/L of urathane was used for anesthesia through abdominal injection in a dose of 125 mg/100 g and a central incision was made along the neck. Trachea and common carotid arteries on both sides were carefully bluntly separated and e xposed.Non-traumatic arterial clamps were used to clamp common carotid arteries and pneumogastric nerves of both sides to induce incomplete cerebral ischemia a nd the clamps were relieved after 60 minutes.Animals were executed after 6-hour of reperfusion and blood and brain were taken immediately for detection.The com mon carotid arties in sham-operation group were not clamped.All above operation s were completed by experienced technicians of our department. MAIN OUTCOME MEASURES:A comparative observation of the impacts of Silybin and SPC of two different doses on water content and pathological changes in cerebral tissues as well as serous MDA content in cerebral ischemic reperfusion model ra ts. RESULTS:①Except for the rats in sham-operation group,cerebral edema occurred in all rats of other groups.In the rats of model group,the brain tissue surface swelled,arachnoid vessels became thicker;some scattered bleeding points were se en.Under microscope,extracellular space increased, basal membrane of capillary vessels loosened,endothelial cells swelled, vacuole enlarged,cytoplasm loosened and the staining in nuclei was light. Neurocyte and glial cells swelled and few vacuoles occurred in cytoplasm,and cell organelles mild enlarged and staining be came light.The observatory results with naked eye of the pathological changes in cerebral tissues in SPC-1 group,SPC-2 group and Silybin group were similar to that of model group but only with very few bleeding points. Under microscope,th e swelling of cells and the increase of extracellular space were relatively mild and relatively fewer vacuoles in cytoplasm.There was no enlargement in cell org anelles in SPC-2 group and the staining in nuclei was similar to that of sham- operation group.②There were differences in water content in brain tissue in eac h gr
出处
《中国临床康复》
CSCD
2004年第22期4629-4631,共3页
Chinese Journal of Clinical Rehabilitation
基金
河南省科技攻关项目(001180203)~~
