摘要
15℃下 ,添加 Ve浓度为 2 0～ 80 ng/ m l时 ,种群密度与培养时间呈显著相关 ,其中 2 0 ng/ ml和 4 0 ng/ ml组最大种群密度分别为 76 .4 0 ind/ ml和 6 5 .2 0 ind/ ml,显著高于对照组 (最大密度 12 .0 0 ind/ ml)。最大混交雌体百分率低于 10 % ,对照组最大混交雌体比率为 4 0 %。轮虫卵雌比有不同程度的提高 ,但维生素浓度为 10 0 ng/ ml时 ,对种群增长不利 ;2 5℃下 ,对照组和各添加组最大密度依次为 6 8.0 0 ,12 2 .0 0 ,12 4 .0 0 ,16 8.0 0和 2 36 .0 0 ind/ ml。最大卵雌比依次为 1.2 1,1.2 5 ,1.2 2 ,1.4 1和 0 .6 9,混交雌体百分比分别为 2 7.6 9,19.89,18.4 2 ,17.0 2和 12 .88% ;30℃下 ,最大密度依次为 113.80 ,12 1.0 0 ,12 2 .80和 15 1.2 0 ind/ ml,最大卵雌比依次为 1.5 0 ,1.4 2 ,0 .6 4和 0 .96。混交雌体百分比分别为 1.76 ,32 .2 6 ,37.5 0和 32 .0 0 %。
The change of population density, the ratio of mictic females and eggs of rotifer Brachionus calyciflorus,cultured in the synthetic medium containing different concentrations of vitamin E,were observed at 15, 25 and 30℃, respectively. Rotifer Brachionus calyciflorus was hatched from resting eggs collected from the sediments in bottom of East Lake (China, Wuhan, 114°23′E,30°33′N). A neonate rotifer hatched from resting egg was transferred into flask containing inorganic medium described by Gilbert (1963). Stock rotifers were fed on Chlorella pyrenoidosa, which was grew in the HB_4 medium with light intensity about 5,000lx provided by 8 cool-white fluorescent lamps (16:8 h light:dark cycle; temperature at 25℃) in the incubator. Before feeding, algae in log phase of growth were harvested, centrifuged at 4,000 rpm for 5 minutes, rinsed with HB_4 medium with 3 times and resuspended in rotifer culture medium. Two neonate rotifers were placed into test tube, each tube containing 10ml of C. pyrenoidosa suspension (2×10~6 cells/ml) diluted with rotifer culture medium. Five different concentrations of vitamin E (0 (as control group), 20,40,80 and 100ng/ml) were added into each test tube, respectively. Light intensity and photoperiod as the same as above described. The experiment was designed at three different temperatures 15, 25 and 30°C. The test rotifers were checked, counted and transferred into new rotifer culture medium every 24 hours. The mictic female, amictic female, mictic egg, amictic egg and male were distinguished by the method described by Paloheimo (1974). At 15℃, the positive correlation between population density and culturing time were presented in 20, 40 and 80 ng/ml, with correlative equation Y=e^((-0.3301+0.5441X)) (r=0.98,p<0.01), Y=e^((-0.2296+0.5002X)) ( r=0.98, p<0.01) and Y=e^( (-0.3700+0.3691X)) (r=0.85,p<0.01), respectively. The maximal density was 76.40 ind./ml in 20 ng/ml group, and 65.20 ind/ml in 40 ng/ml group, but the highest density was only obtained 12 ind./ml in control. Restrain effect was observed in concentration of 100 ng/ml vitamin E at 15℃. The mictic female percentage was remarkable lower in experimental groups than that in control (p<0.01). Eggs ratio was no significant difference between the experimental groups and control. The population density was significantly lower in control than that in experimental groups at 25°C, the correlative equation between density and culture time was Y=e^((1.4579+0.2842X)) (r=0.86, p<0.01), Y=e^((1.3609+0.3253X)) (r=0.88, p<0.01) and Y=e^((1.0166+0.3544X)) (r=0.90,p<0.01) in 40, 80 and 100ng/ml group, respectively. Highest density of rotifer was 68.00, 124.00, 164.60 and 236.20 ind/ml in 20, 40, 80 and 100 ng/ml, respectively. The mictic females percentage was 19.89%,18.42%,17.02% and 12.88% in 20, 40, 80 and 100 ng/ml (27.69% in control), respectively. Maximal eggs ratio was 1.21(control),1.25,1.22,1.41 and 0.69 ( in 20, 40, 80 and 100 ng/ml, respectively). At 30°C,the significantly positive correlation between density and culture time was found, the correlative equation was Y=e^((-0.8151+0.5203X) )(r=0.86,p<0.01), Y=e^((0.4564+0.4628X) )(r=0.95,p<0.01), and Y=e^((-0.1477+0.8539X)) (r=0.92,p<0.01 ), and Y=e^((1.0283+0.4239X)) ( r=0.84,p<0.01) in 20, 40, 80 and 100 ng/ml groups (Y=e^((-0.0268+0.3342X)) (r=0.84, p<0.01), control), respectively. The maximal population density was 113.80,121.00,122.80 and 151.20 ind./ml in groups of 20, 40, 80 and 100 ng/ml (67.20 ind/ml in control), respectively. Meanwhile, the mictic female percentage was 1.76%,32.26%,37.50% and 32% in each experimental groups, respectively. The maximal eggs ratio was 1.50,1.42,0.64 and 0.96 in each experimental groups, respectively.
出处
《生态学报》
CAS
CSCD
北大核心
2004年第8期1663-1670,共8页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目 ( 3 92 70 0 98)~~
关键词
维生素E
萼花臂尾轮虫
密度
卵雌比
混交雌体
vitamin E
Brachionus calyciflorus
population density
egg ratio
mictic female
