摘要
研究了磷钼杂多酸修饰电极对去甲肾上腺素(NE)、肾上腺素(E)和多巴胺(DA)等儿茶酚胺类神经递质的催化氧化作用,探讨了催化机理,并采用高压液相色谱电化学方法对其进行了分离检测,NE、DA和E的线性范围(mol)依次为8.0×10^(-11)～2.0×10^(-8)、8.0×10^(-11)～2.0×10^(-8)、4.0×10^(-11)～2.0×10^(-8);检测限(mol))依次为4.0×10^(-11)、4.0×10^(-11)、2.0×10^(-11).7次平行测定的相对标准偏差(％)依次为1.6、2.0和4.5.将此法用于鼠脑组织中神经递质的测定,获得满意的结果.
An electrode modified with 1 : 12 molybdophosphate (PMo12 CME) was prepared. Its high electrocatalytic effect on the oxidation of catecholamines (noradrenaline(NE), adrenaline (E) and dopamine(DA)) was described, and the mechanism was discussed. LCEC with PMo12 CME was used to separate and detect catecholamines. The linear range of detection is 8. 0×10-11-2. 0×10-8 mol for NE and DA, 4. 0×10-11-2. 0×10-8 mol for E. The detection limit is 4. 0×10-11 mol for NE and DA, 2. 0×10-11 mol for E. The relative standard deviation for seven parallel determination is 1. 6% for NE, 2. 1% for DA and 4. 5% for E. The noradrenaline in rat brain was detected and the results are satisfactory.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1993年第7期914-917,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
液相色谱
去甲肾上腺素
肾上腺素
1 : 12 Molybdophosphate modified electrode
Liquid chromatography electrochemistry
Noradrenaline
Adrenaline
Dopamine
