摘要
以金边蝴蝶兰花梗诱导产生的花梗芽的叶片为外植体,不经过原球茎途径,由叶片直接诱导不定芽进行快速繁殖。比较了叶片在不同培养基上的诱导效果,结果表明:在MS+6-BA5mg/L+NAA1mg/L+CW(椰子水)100mL/L上的诱导率约为32.8%,不定芽在由Hyponex(7-6-19)3g/L+胰蛋白胨1g/L+活性炭1g/L+香蕉泥100g/L+IBA1mg/L+NAA0.5mg/L组成的培养基上生根率为95%。
Adventitious buds of Phalaenopsis Blume were induced directly from lea ves of plantlets of flower stalk culture without the development of protocorm-li ke body (PLB). Results showed that the MS + 6-BA 5 mg/L + NAA 1 mg/L + CW 100 mL/L produced an inducing rate of 32.8% adventitious buds and that the adventitiou s buds produced a rooting rate of 95% when cultured under the hyponex(7-6-19) 3 g/L + tryptone 1 g/L + activated charcoal 1 g/L + banana homogenate 100 g/L + IB A 1 mg/L + NAA 0.5 mg/L.
出处
《热带农业科学》
2004年第3期18-20,69,共4页
Chinese Journal of Tropical Agriculture
关键词
金边蝴蝶兰
叶片培养
不定芽
组织培养
Phalaenopsis Blume
leaves
adventitious buds
tissue culture
