摘要
基于DNA微点阵芯片技术的细菌基因转录谱研究是基因功能分析的重要手段之一。本文阐述了细菌DNA芯片基因转录谱分析的基本问题 ,包括mRNA和双色荧光实验设计 ,详细介绍了目前细菌体内外DNA芯片基因转录谱的具体应用情况 。
出处
《微生物学免疫学进展》
2004年第3期51-57,共7页
Progress In Microbiology and Immunology
参考文献24
-
1Graham MR, Smoot LM, Lux Migliaccio CA, et al. Virulence control in group A Streptococcus by a two-component gene regulatory system:Global expression profiling and in vivo infection modeling[J].Proc Natl Acad Sci 2002, 99(21): 13855-13860.
-
2Voyich JM, Sturdevant DE, Braughton KR, et al. Genome-wide protective response used by group A Streptococcus to evade destruction by human polymorphonuclear leukocytes[J].Proc Natl Acad Sci 2003, 1OO(4):1996-2001 .
-
3Rhodius V, Van Dyk TK, Gross C, et al. Impact of genomic technologies on studies of bacterial gene expression[J].Annu. Rev.Microbiol 2002, 56: 599-624.
-
4Moody DE. Genomics techniques: An overview of methods for the study of gene expression[J]. J Anim Sci (Suppl.) 2001, 79:E128-E135.
-
5Qing X, Michelle D, John JM. Determination of the transcriptome of Vibrio cholerae during intraintestinal growth and midexponential phase in vitro[J].Proc Natl Acad Sci 2003, 100(23): 1286-1291 .
-
6Guido D, Sebastian K, Claudia H, et al. Transcriptome Analysis of Neisseria meningitidis during infection[J].J Bacteriol 2003,185:155-164.
-
7Eriksson S, Lucchini S, Thompson A, et al. Unravelling the biology of macrophage infection by gene expression profiling of intracellular Salmonella enterica[J].Molecular Microbiology 2003,47 (1):103-118.
-
8Ye RW, Wang T, Bedzyk L, et al. Applications of DNA microarays in microbial systems[J].J Microbiol Methods 2001,47: 257-272.
-
9Schoolnik GK. Microarray analysis of bacterial pathogenicity[J].Adv Microb Physiol 2002,46:1-45.
-
10Schoolnik GK. Functional and comparative genomics of pathogenic bacteriar[J].Current Opinion in Microbiology 2002, 5:20-26.
同被引文献19
-
1陆承平.猪链球菌病与猪链球菌2型[J].科技导报,2005,23(9):9-10. 被引量：27
-
2庞昕,韩延平,周冬生,宋亚军,张玲,翟俊辉,王津,郭兆彪,杨瑞馥.基于DNA芯片的细菌基因表达谱技术的建立与评价[J].中华微生物学和免疫学杂志,2006,26(2):179-184. 被引量：2
-
3Yu H,Jing H,Chen Z,et al.Human Streptococcus suis outbreak,Sichusn,China[J].Emerge Infect Dis,2006,12(6):914-920.
-
4卫生部通报四川省人感染猪链球菌病疫情[EB/OL].http://www.moh.gov.cn/news,2005-08-03.
-
5冯友军,高福.猪链球菌2型研究:一种新病型的发现及其致病机制的探索[D].北京:中国科学院微生物研究所,1998.
-
6Vecht U,Wisselink H J,Jellema M L,et al.Identification of two proteins associated with virulence of Streptococcus suis type 2[J].Infect Immun,1991,59(9):3156-3162.
-
7Gottschalk M G,Lacouture S,Dubreuil J D.Characterization of Streptococeus suis capsular type 2 haemolysin[J].Microbiology,1995,141(1):189-195.
-
8Winterhoff N,Goethe R,Gruening P,et al.Identification and characterization of two temperature-Induced surface-associated proteins of Streptococcus suis with high homologies to members of the Arginine Deiminase System of Streptococcus pyogenes[J].JB,2002,184(26):6768-6776.
-
9Conway T,Schoolnik G K Micrcarray expression profiling:capturing a genome-wide portrait of the transcriptome[J].Mol Microbiol,2003,47(4):879-889.
-
10Han Y,Zhou D,Pang X,et al.Microarray analysis of temperature-induced tranacriptome of Yersinia pestis[J].Microbiol Immunol,2004,48:791-805.
二级引证文献2
-
1王中胜,曾小涛,袁媛,尉研,郑玉玲,姜永强.猪链球菌2型强毒株05ZYH33转录调控因子Rgg基因敲除突变体与野生株的基因表达差异分析[J].生物技术通讯,2010,21(6):812-817. 被引量：1
-
2尉研,袁媛,曾小涛,江华,郑玉玲,周冬生,姜永强.猪链球菌2型强毒株S.suis 05ZY和弱毒株S.suis 1940的比较转录谱分析[J].生物技术通讯,2012,23(1):11-14.
-
1周家喜.基因芯片技术及其在生殖生物学中的初步应用[J].中国计划生育学杂志,2002,10(7):446-447. 被引量：1
-
2周冬生,杨瑞馥,宋亚军,庞昕,翟俊辉.DNA芯片技术在微生物研究中的应用[J].军事医学科学院院刊,2002,26(4):294-300. 被引量：4
-
3魏小勇,罗灼玲,李彩霞.组织芯片联合基因芯片在肿瘤研究中的应用[J].解剖科学进展,2004,10(4):368-370. 被引量：1
-
4郑义,罗海波,高博,黄来强.丙型肝炎病毒非结构蛋白NS4B抑制IFN介导的抗病毒反应[J].微生物学报,2006,46(5):802-806. 被引量：2
-
5吴彬,周淑芸,刘晓力.基因芯片技术在恶性血液系统疾病研究中的应用[J].国外医学（内科学分册）,2001,28(6):234-236.
-
6张志祥,沈铮,赵辉,李宾,宋世平,胡钧,林炳承,李民乾.蛋白质DNA混合微点阵和微流控芯片的整合[J].化学学报,2005,63(18):1743-1746.
-
7李远森,马志杰,田淑琴.DNA芯片技术在新兽药开发中的应用现状与展望[J].西南民族大学学报（自然科学版）,2003,29(3):360-362.
