摘要
目的 :探讨 RT- PCR检测胃癌区域淋巴结微转移的价值及其应用前景。方法 :以病理组织学和 RT- PCR扩增 K19对 30例胃癌患者的癌组织标本及 12 6个淋巴结进行检测。结果 :30例胃癌患者癌组织均有 K19m RNA表达 ;12 6个淋巴结中 ,组织学阳性 2 6个 (2 0 .6 % ) ,而 RT- PCR阳性 4 2个 (33.3% ) ,RT- PCR扩增 K19及内参基因β- actin后 ,所有瘤组织、转移淋巴结显示 4 6 0和 5 4 0 bp的扩增片段 ,非肿瘤患者的淋巴结中仅显示 5 4 0 bp。结论 :RT- PCR扩增 K19m RNA检测胃癌区域淋巴结微转移是一种敏感、特异的检测方法 ,优于常规病理组织学。
Objective To study the detection of gastric cancer micrometastasis in regional lymph nodes by reverse transcriptase polymerase chain reaction (RT-PCR)and its promising application. Methods Tumor tissues of 30 cases of gastric cancer and 126 lymph nodes were studied by both histopathological examination and K19 mRNA RT-PCR. Results K19 mRNA expressed in all specimens of 30 patients with gastric cancer, 26 examined lymph nodes showed postive lymph node metastasis by routine histopathological examination (20.6%), but the number of postive lymph nodes detected by RT-PCR increased to 42 (33.3%). After K19 and β-actin were amplificated by RT-PCR, the amplification fragments of 460 and 540 bp appeared in all cancer and gastrointestinal tissues. Conclusion K19mRNA detection by RT-PCR is superior to routine histpathological examination and is a highly sensitive and specific method.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第4期577-579,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题 (2 0 0 0 0 32 7)
关键词
胃肿瘤
逆转录聚合酶链反应
角蛋白19
淋巴结
微转移
stomach neoplasms
reverse transcriptase polymerase chain reaction
K19
lymph nodes
micrometastasis
