摘要
目的 为了进一步明确铝及含铝化合物对神经系统的损伤及其作用机制。方法 采用全细胞膜片钳技术研究三氯化铝 (AlCl3)对急性分离的大鼠海马CA1区神经元钠通道的影响。结果 AlCl3对钠电流有明显的抑制作用 ,且呈浓度依赖性 ,10 0 0μmol·L- 1AlCl3给药前后钠电流激活曲线Vh 分别为- (5 1.3± 6 .0 )mV和 - (4 7.5± 5 .4 )mV (P <0 .0 5 ) ,k值分别为 - (8.1± 2 .3)mV和 - (8.6± 3.2 )mV(P >0 .0 5 ) ,给药前后钠电流失活曲线Vh 分别为- (6 7.4± 5 .5 )mV和 - (71.4± 4 .4 )mV(P <0 .0 5 ) ,k值分别为 (6 .1± 1.1)mV和 (6 .8± 1.2 )mV (P >0 .0 5 )。结论 AlCl3对大鼠海马CA1区神经元钠通道有抑制作用 。
AIM To further explore the damage effect of aluminum and compounds containing aluminum on nervous system and its mechanisms. METHODS The effects of aluminum chloride (AlCl 3) on Na + currents of isolated hippocampal CA1 neurons of rats were studied using whole cell patch clamp technique. RESULTS AlCl 3 could reduce Na + current in a concentration dependent manner. Before and after application of 1000 μmol·L -1 AlCl 3, the half activation voltage of Na + current were -(51.3±6.0)mV and -(47.5±5.4) mV (P<0.05), but the slope was not changed. Before and after application of 1000 μmol·L -1 AlCl 3, the half inactivation voltage of Na + currents were -(67.4±5.5)mV and -(71.4±4.4)mV(P<0.05), but the slope was not changed. CONCLUSION AlCl 3 may damage sodium channels of the hippocampal CA1 neurons of rats, which may be related to the mechanism of damage of aluminum on central nervous system.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2004年第4期309-312,共4页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金资助项目(30070647)~~
关键词
铝
海马
膜片钳技术
全细胞
钠通道
aluminum
hippocampus
patch clamp technique, whole cell
sodium channels
