摘要
目的 :采用高含量胆固醇建立动脉粥样硬化性阴茎勃起功能障碍 (ED)动物模型并探讨其诱发ED机制。 方法 :30只雄性新西兰大白兔 ,随机分为 2组 :普通饲料 (ND)组 1 0只 ,喂以普通饲料 ;高胆固醇组 2 0只 ,喂以含 1 .5 %胆固醇的饲料。处理 1 2周后观测血脂水平、升主动脉斑块和阴茎勃起情况 ,测定阴部内动脉内膜 /中膜厚度比值 ,以及RT PCR法检测阴部内动脉中血管细胞粘附分子 (VCAM 1 )mRNA表达水平。 结果 :实验过程中共有 5只新西兰大白兔死亡 ,高胆固醇组 3只 ,对照组 2只。喂饲含 1 .5 %胆固醇饲料的新西兰大白兔 1 2周后均出现高脂血症 ,其中 9只发生典型的动脉粥样硬化 (为ATH组 ) ,动脉粥样硬化诱导成功率为 5 2 .9%。ATH组和HCH组 (喂饲高胆固醇但未发生典型动脉粥样硬化者 )TC、TG、AI均显著高于ND组 (P <0 .0 1 )。ATH和HCH组新西兰大白兔阴茎勃起率及阴茎勃起次数均明显降低 ,其中尤以ATH组降低更显著 (P <0 .0 1 )。ATH组阴部内动脉内膜 /中膜厚度比值和VCAM 1mRNA表达水平显著高于HCH和ND组 (P <0 .0 1 )。 结论 :采用高含量胆固醇喂饲新西兰大白兔建立ED动物模型较为简便、可行可靠。高胆固醇血症可能通过VCAM 1损害动脉内皮细胞功能 ,造成动脉内膜增厚。
Objective: To establish the animal model of atherosclerotic erectile dysfunction induced by high cholesterol diet and explore the mechanisms of atherosclerotic erectile dysfunction. Methods: Thirty male rabbits were divided into two groups randomly:normal diet(ND) group( n =10)and high cholesterol(HCH)group fed with 1.5 % cholesterol diet( n =20). Serum total cholesterol, plaque areas of the ascending aorta, the ratio of intima/media thickness and level of vascular cell adhesion molecule 1(VCAM 1) mRNA expression of internal pudendal artery were determinated after twelve weeks. Results: After twelve weeks, all rabbits of the HCH group got hyperlipemia, 9 of which presented typical atherosclerosis(ATH). The rate of atherosclerosis induced by high cholesterol diet was 52.9 %.Serum total cholesterol levels of the ATH and HCH groups were higher than that of the ND group( P < 0.01 ). The penile erection times and rate in the ATH and HCH groups decreased significantly, compared with the ND group( P < 0.01 ). Both the ratio of intima/media thickness and the level of VCAM 1mRNA expression of internal pudendal artery in the ATH group were higher than those in the HCH and ND groups( P < 0.01 ). Conclusion: It is easy, prectuable and reliable to establish the animal model of atherosclerotic erectile dysfunction induced by high cholesterol diet. And one of the main causes of atherosclerotic erectile dysfunction is hypercholesterolemia, which through VCAM 1 may induce the structural and functional modifications of the endothelium of the internal pudendal artery and make the corpus cavernosum ischemia.
出处
《中华男科学杂志》
CAS
CSCD
2004年第8期608-611,共4页
National Journal of Andrology
