摘要
目的 探讨经兔肝动脉灌注As2 O3对肝移植瘤的抑制作用。方法 采用不同浓度As2 O3经肝动脉插管灌注治疗兔肝脏Vx 2移植瘤 ,连续 7d ,观察肿瘤平均质量、平均瘤重抑制率、肿瘤细胞形态学变化 ,检测bcl 2 bax基因表达和VEGF表达。结果 实验组的平均瘤重分别为 7 99、6 5 0、4 87g ,平均瘤重抑制率分别为 5 0 3 1%、5 9 5 8%和 69 71%,实验组移植瘤瘤重低于阴性对照组 (P <0 .0 5 )。随着As2 O3的剂量增加 ,实验组的瘤重减轻而平均瘤重抑制率增加 (P <0 .0 5 )。实验组内移植瘤组织bax基因表达显著上调 ,bcl 2基因表达、VEGF表达显著下调 (P <0 .0 5 )。而bcl 2基因、VEGF表达下调无差异性(P =1 0 0 )。电镜观察实验组各组均有典型的瘤细胞凋亡形态学改变。结论 经肝动脉灌注As2 O3治疗兔Vx 2肝移植瘤 ,有显著的抗肿瘤作用 ,并具有剂量依赖特点。
Objective To evaluate the anticancer effect of arsenic trioxide (As 2O 3) on rabbits with hepatic Vx 2 carcinoma by transcatheter arterial infusion (TAI). Methods Rabbits with hepatic Vx 2 carcinoma were treated with As 2O 3 by TAI for 7 d consecutively. The tumor inhibitory rate and the average tumor weight were determined. The morphological changes of the tumor were observed with a transmission electron microscope. bax/bcl 2 and VEGF expressions were examined by immunohistochemistry. Results The average weight of the tumor was 7.99, 6.50, and 4.87 g, and the tumor growth inhibitory rates in the experimental groups were 50.31%, 59 58%, and 69 71%, respectively. Tumor growth was significantly inhibited as compared with that in the NS group ( P <0.05). The upregulated bax expression and downregulated bcl 2 expression were detected in As 2O 3 groups by immunohistochemistry. There was significant difference between the As 2O 3 groups and the control group ( P <0.05). Significantly decreased VEGF expression was also observed in As 2O 3 groups as compared with the controls ( P <0.05). Bcl 2 gene expression was consistent with VEGF expression. Transmission electron microscopy showed typical morphological characteristics of apoptosis of some tumor cells in the experimental groups. Conclusion As 2O 3 by TAI can inhibit rabbit hepatic Vx 2 carcinoma effectively in a dose dependent manner. Apoptosis induced by As 2O 3 may be closely related to the upregulated bax expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第16期1436-1438,共3页
Journal of Third Military Medical University
关键词
三氧化二砷
动脉灌注
肝脏
移植
肿瘤
arsenic trioxide
transcatheter arterial infusion
liver
Vx 2 carcinoma
rabbit
