摘要
目的 探讨促炎症细胞因子 :肿瘤坏死因子 α(TNF α)、白细胞介素 1β(IL 1β)和白细胞介素 6(IL 6)水平的变化 ,在全身炎症反应和急性肺损伤中的作用。方法 采用油酸加内毒素脂多糖 (LPS)序贯致伤大鼠 ,在油酸致伤后 1、4、12和 2 4h实施小剂量LPS第 2次致伤 ,收集血浆和肺泡灌洗液 ,采用酶联免疫法 (ELISA)检测TNF α、IL 1β和IL 6蛋白含量。以单油酸致伤和单小剂量LPS致伤作为对照组进行比较分析。结果 TNF α和IL 1β在油酸致伤 4hLPS第 2次致伤后水平最高 ,IL 6的最高水平在油酸 12h加LPS致伤组 ;2次序贯致伤后IL 1β水平变化最大 ,增高最显著 ,血浆浓度达到 ( 15 2 6± 3 9.69)pg ml ,肺泡灌洗液内浓度为 ( 840 .4± 95 .2 9)pg ml。结合肺组织病理学研究 ,肺组织损伤最严重大鼠 ,血浆和肺泡灌洗液TNF α、IL 1β和IL 6水平最高。 结论 TNF α和IL 1β是早期释放的促炎症细胞因子 ,其中IL 1β的血浆和肺泡灌洗液中水平最高 ,它可能在全身炎症反应发生、发展和急性肺损伤中起非常重要的作用。
Objective To study the roles of pro inflammatory cytokines tumor necrosis factor α (TNF α), interleukin 1β (IL 1β), and interleukin 6 (IL 6) in acute lung injury and systemic inflammatory response syndrome (SIRS). Methods Enzyme linked immunosorbent assay (ELISA) was employed to determine the protein concentrations of TNF α, IL 1β, and IL 6 in the plasma and bronchoalveolar lavage fluid (BALF) of rats after seguential injury with oleic acid and lipopolysaccharide at different time. Results The peak levels of TNF α and IL 1β were found at 4 h after first hit with oleic acid. After second hit, more severe pathologic changes were found in the lung tissues, and higher protein concentrations of TNF α, IL 1β, and IL 6 were also found in the plasma and BALF. Conclusion TNF α and IL 1β are present in the early phase of ALI. IL 6 may play an important role in the delayed development. Increased release of pro inflammatory cytokines, such as TNF α, IL 1β, and IL 6, may be one of the mechanisms in the development of ARDS and SIRS.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第15期1354-1356,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助重点项目 ( 39730 2 10 )~~
