摘要
目的:探讨2型糖尿病(NIDDM)患者中肿瘤坏死因子(tumor necrosis factor,TNFβ)基因多态性与2型糖尿病相关性。方法:利用聚合酶链式反应技术对59例2型糖尿病患者及103例正常对照者的肿瘤坏死因子基因进行扩增以cfoI进行限制性酶切图谱分析。结果:正常人群和NIDDM人群中TNFβ基因型均以TNFβ1/2型最为多见,TNFβ基因型分布和等位基因频率在正常和NIDDM人群组间和组内比较均无显著差异(P>0.05)。结论:TNF基因多态性可能在NIDDM及代谢紊乱的发生发展中不起重要作用。
Objective: To study the relationship of TNFβ polymorphism with type 2 diabetes mellitus. Method: the genotypes of TNFβ were determined by PCR-based assay in 59 patients with type 2 diabetes mellitus and 103 healthy controls. Results: there were no siganificant differences in the frequenceies of TNFβ mutations between the patients and control groups. Conclusions: The TNFβ mutation is not associated with type 2 diabetes mellitus.
出处
《广州医学院学报》
2004年第2期48-49,52,共3页
Academic Journal of Guangzhou Medical College
