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野生型p53基因转染前列腺癌细胞系PC-3m对细胞凋亡的不同影响 被引量：2

Effect of transient transfection of wild-type p53 gene on the proliferation and apoptosis of prostate cancer cell PC-3m
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摘要 目的:观察野生型p53基因转染对前列腺癌细胞PC鄄3m增殖和凋亡的影响。方法:用脂质体Lipofectamine将真核表达载体pCMV鄄p53和pCMV鄄p53mt135分别转染PC鄄3m细胞,MTT法检测转染前后细胞生长情况,转染48h后采用流式细胞技术(FCM)检测细胞凋亡率。结果:pCMV鄄p53转染后的PC鄄3m细胞生长明显受到抑制,FCM显示,转染48h后,细胞凋亡率为15%。结论:野生型p53基因瞬间转染PC鄄3m细胞可抑制细胞增殖,诱导细胞凋亡。 Objective: To observe the effect of transient transfection of wild-type p53 gene on the proliferation and apoptosis of prostate cancer cell line PC-3m. Methods: The eukaryotic expression vector pCMV-p53 was transfeced into PC-3m cells with lipofectamine. The cell growth was observed before and after transfection by MTT assay. The cells were collected 48 hours after transfection for determining apoptotic rate by flow cytometric (FCM) analysis. Results: The PC-3m cell growth after transfection was inhibited by MTT. The apoptotic rate was 15% 48 hours after transfection. Conclusion: The transient transfection of wild-type p53 gene induces the apoptosis of prostate cancer cell line PC-3m.
作者 贾立宁 张莲英 张建业 孔峰 胡晓燕 陈蔚文
机构地区 山东大学医学院生化与分子生物学研究所
出处 《山东大学学报（医学版）》 CAS 2004年第4期380-383,共4页 Journal of Shandong University:Health Sciences
基金 国家自然科学基金项目(30171026) 山东省卫生厅基金项目(99CAICAA2)
关键词 基因 P53 前列腺肿瘤 细胞凋亡 Gene,p53 Prostatic neoplasms Apoptosis
分类号 R737.25 [医药卫生—肿瘤]
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参考文献14

  • 1Merritt AJ, Potten C, Kemp C, et al. The role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53 deficient mice [J]. Cancer Res,1994,54(3):614-617.
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二级参考文献6

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山东大学学报（医学版）
2004年 第4期

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