摘要
目的 :探讨红细胞生成素受体 (EPO R)在白血病细胞上的表达及其意义 ,以及重组人EPO(rhEPO)对急性白血病细胞增殖的影响。方法 :应用独特型EPO R单克隆抗体 ,通过流式细胞仪技术检测 30例急性白血病 (AL)患者的白血病细胞EPO R的表达 ,用细胞周期DNA分析法研究rhEPO单独对AL细胞增殖的影响。结果 :30例AL患者白血病细胞均有EPO R表达 ,EPO R表达率 (4 .6± 4 .2 ) %明显低于作为对照组 ,P <0 .0 5。 18例AL患者的白血病细胞体外经rhEPO作用后未出现明显的增殖 ,与对照组比较 ,无显著性差异 (P >0 .0 5 )。结论 :EPO R在AL细胞中均有表达 ,但表达水平较低。rhEPO体外不刺激AL细胞的增殖。EP0 R在AL中的作用、生物学特性及与预后的关系还有待于深入研究。
OBJECTIVE To study the expression of erythropoietin receptor(EPO-R) in leukemic cells and its implication and investigate the effect of recombinant human erythopoietin(rhEPO) on leukemic cells. METHODS EPO-R was detected in 30 AL patients by using idio-monoclonal antibody and flow cytometry as well as the effect of rhEPO on acute leukemic cells by DNA assay. RESULTS EPO-R was expressed in all 30 AL patients. The expressive rafe of EPO-R(_4.6±_4.2)% was significant lowr than that of control group(P<_0.05). The treatment with rhEPO in vitro did not induced significantly proliferation of leukemic cells in 18 AL patients,and there was no relationship between the proliferation of leukemia cells and the expressive level of EPO-R. CONCLUSION EPO-R was expressed in AL,but the expressive level was low and did not affect the CR rate in AL.rhEPO could not stimulate AL cells to proliferate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第9期529-531,共3页
Chinese Journal of Hospital Pharmacy
