摘要
目的 :探讨急性白血病 (AL)骨髓血管新生的状态及意义。方法 :应用改良的GMA塑料包埋骨髓病理切片进行免疫组化染色检测AL患者治疗前后骨髓微血管密度 (MVD) ,应用ELISA方法检测AL患者治疗前后血清血管内皮生长因子 (VEGF)的浓度。结果 :AL患者治疗前骨髓MVD明显增加 ,化疗缓解后骨髓MVD降至正常水平 ,复发者骨髓MVD再次升高至化疗前水平 ;AL患者治疗前血清VEGF水平比正常对照明显升高 ,化疗缓解的患者VEGF水平仍然较高。结论 :骨髓血管新生在AL发生过程中有着重要的作用 。
Objective:To investigate the state and significance of bone marrow angiogenesis of hematological diseases.Method:Bone marrow microvascular density (MVD) in plastic-embedded section was examined using acetone-fixed bone marrow tissues embedded in glycol-methacrylate (GMA) resin by the method of immunohistochemistry. Serum levels of vascular endothelial growth factor (VEGF) were examined by the method of ELISA.Result:The study showed that bone marrow MVD increased greatly in newly diagnosed acute leukemia if no treatment was provided. Bone marrow MVD decreased to the same range as the controls at the time of complete remission.That of the relapsed increased again to the same range as the newly diagnosed acute leukemia. Serum levels of VEGF of patients with newly diagnosed acute leukemia increased greatly compared with those of the controls, but those of the complete remission are significantly elevated compared with the controls and double times higher than the controls.Conclusion:These observations suggested that bone marrow angiogenesis might play a key role in the pathogenesis and development of acute leukemia. Serum VEGF levels could result in bone marrow angiogenesis in acute leukemia to some extent.There was not correlation between serum VEGF levels and bone marrow MVD.
出处
《临床血液学杂志》
CAS
2004年第5期254-256,共3页
Journal of Clinical Hematology
关键词
白血病
急性
骨髓微血管密度
血管内皮生长因子
acute leukemia
bone marrow microvascular density
vascular endothelial growth factor
