摘要
目的 :观察侧脑室注射 (intra cerebroventricularinjection ,i.c .v)纳洛酮 (naloxonehydrochloride ,NAL)对反复脑缺血再灌注小鼠学习记忆的影响及机制。方法 :小鼠作侧脑室埋植套管 ,术后抗感染 4～ 5d ,然后采用清醒小鼠造成反复脑缺血再灌注模型 ,电迷宫法观察小鼠学习记忆情况 ,比色法检测脑内丙二醛含量 ,放射免疫法测定脑内及血浆内 β 内啡呔(β endorphin ,β Ep)含量。 结果 :纳洛酮可以明显改善反复脑缺血再灌注小鼠学习记忆的情况 ,同时可不同程度地抑制由反复脑缺血再灌注引起的脑内异常升高的丙二醛、β 内啡呔 ,改善海马CA1区细胞数目。结论 :纳洛酮对反复脑缺血再灌注损伤有保护作用 ,其机制可能与抑制脑内升高的丙二醛、β 内啡呔 ,从而提高海马CA1区细胞数目有关。
AIM: To observe the effects and mechanism of naloxone being injected intra-cere broventricular on studying and memory in mice with repeatedly cerebral ischemia reperfusion. METHODS: Double-tube was embedded in lateral ventricle of mice, anti-infection was taken for 4 or 5 days to mice after operation, and the repeated cerebral ischemia reperfusion model was made in awakening mice. Behavior of learning and memory was observed by eletic maze test, methylenedioxy (MDA) in brain tissue was detected by TBA, the content of β-Ep in brain tissue and blood plasma was measured by radio-immunity method, and the number of cell was counted in htppcoampal CA1 on section of brain tissue. RESULTS: Naloxone obviously improved the disorder of studying and memory of mice, and inhibited the abnormal increased MDA and β-Ep in brain tissue caused by repeated cerebral ischemia. It also improved the number of cell in htppcoampal CA1. CONCLUTION: Naloxone can protect mice being repeatedly cerebral ischemia reperfusion from the damage of studying and memory, and its mechanism is related to its contradicting β-Ep activity and lowing MDA in brain and increased htppcoampal CA1.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第8期935-938,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
侧脑室注射
反复脑缺血再灌
学习记忆
纳洛酮
丙二醛
Β-内啡呔
intra-cerebroventricular injection
repeated cerebral ischemia-reperfusion
naloxone hydrochloride
studying and memory
malonydialdehyde (MDA)
β-endorphin
htppcoampal CA1
