摘要
介绍了一种适用于多种真菌染色体DNA的快速提取方法。该方法用石英砂振荡破壁 ,快速便捷地提取真菌染色体DNA ,提取时间仅用 1～ 2h。作者应用该方法成功地提取了粗糙脉胞菌 (Neurosporacrassa)、米曲霉 (Aspergillusoryzae)、羊肚菌 (Morchellaesculcnta)、酿酒酵母 (Saccharomycescervisae)等 4种不同真菌的染色体DNA ,所提DNA片段均大于2 0kb ,可直接用于限制性内切酶酶切。
The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1～2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with restriction endoenzyme or PCR
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第4期89-92,共4页
Microbiology China
