摘要
建立高效液相色谱法同时测定复痤霜中克林霉素和螺内酯的含量。采用C18柱,流动相为乙腈-磷酸盐缓冲液(pH7.4)(45∶55),检测波长214nm。克林霉素和螺内酯均在30～70靏/ml范围内线性关系良好(r>0.999),回收率大于98%,日内、日间RSD均小于2%。
A HPLC method for simultaneous determination of clindamycin and spironolactone in compound clindamycincream was established. A C18 column was used with the mobile phase of acetonitrile-phosphate buffer solution(pH 7.4)(45∶55) at the detection wavelength of 214nm. The calibration curves of clindamycin and spironolactone were linear inthe concentration range of 30～70ìg/ml(r>0.999). The recoveries were more than 98%. The RSDs of intra- and inter-day were less than 2%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2004年第9期542-544,共3页
Chinese Journal of Pharmaceuticals
