摘要
目的 克隆人PD L2基因并构建PD L2胞外区的原核表达载体 ,在大肠杆菌中进行表达。方法 以RT PCR方法从活化的人外周血单个核细胞总RNA中克隆PD L2基因的cDNA ,构建PD L2胞外区的原核表达载体 ,在大肠杆菌BL2 1(ED3)中进行表达并鉴定。结果 克隆到PD L2基因cDNA编码区全长序列 ,经DNA测序证明其与已报道的序列一致。进而构建了PD L2胞外区的原核表达载体 ,并在大肠杆菌表达 ,免疫印迹分析表明在IPTG诱导后表达PD L2胞外区蛋白 ,相对分子质量Mr 为 2 2 0 0 0 ,与理论值大小相符。结论 成功克隆PD L2基因 ,其胞外区蛋白在大肠杆菌中获得表达 ,为进一步研究PD L2功能提供了条件。
Objective To clone the human PD-L2 gene and to construct a prokaryotic expression vector for the extracellular domain of PD-L2. Methods The cDNA of human PD-L2 gene was cloned from the total RNA of activated human peripheral blood mononuclear cells by RT-PCR. The prokaryotic expression vector for the extracellular domain of PD-L2 was constructed and its expression in Escherichia coli (E. coli) was determined. Results The full-length coding sequence of PD-L2 gene was cloned and confirmed by DNA sequencing, which was identical to the published gene. The prokaryotic expression vector for the extracellular domain of PD-L2 was then constructed. The recombinant protein was expressed in E. coli after the IPTG induction and identified by Western blotting. The recombinant protein had a molecular weight of 22 000, which was consistent with theoretical calculation. Conclusion The gene of PD-L2 is successfully cloned and the extracellular domain protein of the PD-L2 is expressed in E. coli, which provides a basis for the further study of the function of PD-L2.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第5期346-349,共4页
Immunological Journal
基金
国家自然科学基金重点项目 (30 2 30 350 )
国家自然科学基金项目 (30 371 651 )
国家重点基础研究发展规划 (973)项目 (G2 0 0 0 570 0 6)资助
