期刊文献+

辣椒叶片RNA提取方法研究 被引量:8

Comparison of RNA extraction from pepper leaves
下载PDF
导出
摘要 以经过紫外线诱导处理的辣椒叶片为材料,分别用Trizol试剂快速提取法、尿素提取法和酚 SDS提取法提取其总RNA,比较各RNA产率、纯度及电泳图谱等,结果表明,尿素法提取的RNA28S和18S条带较为清晰,还可得到23S和16S带,较少有降解;而另2种方法所获得的RNA纯度较低,降解严重,琼脂糖电泳图谱通常只出现1条带. The pepper leaves treated with ultraviolet ray were taken as materials and their total RNA was extracted with Trizole reagent, urea and phenol-SDS, respectively. The producing rates, purities and electrophoretograms of RNA were compared. The RNA extracted with urea produced two clear bands of 28S and 18S, and the bands 23S and 16S could also be obtained with little degradation. Whereas, the RNA extracted with the other two methods were of low purity and much degradation, in which only one band appeared in the agarose gel electrophoretogram usually.
出处 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2004年第3期336-338,共3页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金 福建省自然科学基金资助项目(B0110016).
关键词 辣椒 叶片RNA 提取 紫外线 pepper ultraviolet ray RNA extraction
  • 相关文献

参考文献5

  • 1SAMBROOK J, MANIATIS T, FRITSCH E F. Molecular Cloning[M]. New York:Cold Spring Harbor Laboratory Press,1989.343-36.
  • 2WANG C S,VODKIN L O.Extraction of RNA from tissues containing high levels of procyanidins that bind RNA[J]. Plant Mol Biol Rep, 1994,12(2):132-145.
  • 3夏兰芹,郭三堆.棉花RNA的快速提取方法[J].棉花学报,2000,12(4):205-207. 被引量:56
  • 4赵双宜,吴耀荣,夏光敏.介绍一种简单高效的植物总RNA提取方法[J].遗传,2002,24(3):337-338. 被引量:72
  • 5FANG G, HAMMAR S, GRUMET R. A quick and inexpensive method for removing polysaccharides from plant genomic DNA[J]. Biotechniques,1992,13:52-56.

二级参考文献2

  • 1郭三堆,两种编码杀虫蛋白基因和双价融合表达载体及其应用 [P],980 2 885.3,1996年
  • 2萨姆布鲁克 J,分子克隆实验指南(第2版),1992年,343页

共引文献121

同被引文献75

引证文献8

二级引证文献31

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部