摘要
通过扫描电镜观察和测定生物被膜生长曲线的研究得出 :在不锈钢表面形成的金黄色葡萄球菌生物被膜约 6h后进入稳定期 ,培养 5d后其细菌密度达到 3× 10 6cfu·cm-2 ;金黄色葡萄球菌以生物被膜的形式比以浮游形式生长对二氧化氯具有更强的抵抗能力 ;胰酶大豆肉汤 (tryptonesoybroth ,TSB)
Biofilm formation characteristics of Staphylococcus aureus AS 1 89 on stainless steel surface were observed with scanning electronic microscope (SEM) and measured through its growth curve. The sensitivity of biofilm cells and culture suspension to chloride dioxide and the effect of tryptone soy broth (TSB) on their sensitivity during sterilizing were also studied. The results showed that S. aureus biofilm on stainless steel surface reached to stationary phase after 6?h incubation. The cell density was 3×10 6 cfu cm -2 at 5?d. The bacterial cells on the biofilm had higher resistance to chlorine dioxide than those in suspension. TSB obviously reduced the sterilization effect of chloride dioxide.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2004年第4期10-13,共4页
Journal of China Agricultural University
基金
国家质量检验检疫总局资助项目 ( 2 0 0 3IK0 5 2 )
关键词
扫描电镜
生物被膜
金黄色葡萄球菌
不锈钢表面
scanning electronic microscope
biofilm
Staphylococcus aureus
stainless steel surface
