摘要
目的 研究HIV AIDS患者外周血CD4 + 、CD8+ 淋巴细胞数在不同条件下 (时间、温度和处理过程 )的变化。方法 选取HIV AIDS患者 34例 ,用流式细胞术检测在 4℃条件下放置不同时间(2、2 4、4 8、72h)的外周血CD4 + 、CD8+ 细胞数的变化 ,对经过处理的外周血 (处理过的血样 )CD4 + 、CD8+ 细胞数的变化进行比较 ;对室温条件下放置不同时间 (2、2 4、4 8、72h)处理过的血样CD4 + 、CD8+细胞数的变化进行比较。结果 在 4℃时 ,全血放置 2、2 4、4 8、72h的CD4 + 细胞计数差异无显著意义(P >0 0 5 ) ,而CD8+ 细胞数放置 72h时则差异有显著意义 (P <0 0 5 ) ;处理过的样品放置 72hCD4 +细胞计数差异才有显著意义 (P <0 0 5 ) ,而CD8+ 细胞数在 2 4h时差异就有显著意义 (P <0 0 5 )。在室温时 ,处理过血样放置 2、2 4、4 8、72h的CD4 + 细胞计数差异无显著意义 (P >0 0 5 ) ,而CD8+ 细胞数在 4 8h则差异有显著意义 (P <0 0 5 )。结论 抗凝全血在 4℃放置 4 8h ,检测CD4 + 、CD8+ 淋巴细胞数 ,结果是可靠的。处理过血样在室温放置 2 4h ,检测CD4 + 、CD8+ 淋巴细胞数 ,结果是可靠的。 2 4～4 8h虽然CD4 + 淋巴细胞没有变化 ,但CD8+ 淋巴细胞却发生明显的变化 ,两者比例必然发生变化。
Objective By analyzing the CD4 + and CD8 + T lymphocyte count of whole blood from HIV/AIDS patients,which were stored at different temperatures for various durations,the authors studied the ideal preserving condition for whole blood and processed,in a purpose of guaranteeing the accuracy of clinical testing of CD4 + and CD8 + T lymphocyte count. Methods Blood from 34 HIV carriersl/AIDS patients,were kept at 4℃ for 2,24,48,or 72 h,and tested for CD4 + and CD8 + T lymphocyte count using cytometric analysis. Part of the blood was processed,and kept at 4℃ or room temperature for 2,24,48,or 72 h,then tested for CD4 + and CD8 + T lymphocyte count. The results were compared statistically in parallel. Results Whole blood and processed samples preserved at 4℃ showed no statistical difference in CD4 + T lymphocyte count among different preserving durations ( P >0.05),but CD8 + T lymphocyte counts were significantly different at 72 h ( P <0.05). Processed samples at 72 h were significantly different in CD4 + T lymphocyte count( P <0.05),and significantly different in CD8 + T lymphocyte count at 24 h ( P <0.05).At room temperature,samples at different duration were not significantly different in CD4 + T lymphocyte count,but significantly different in CD8 + T lymphocyte count at 48 and 72 h ( P <0.05). Conclusion There were stable results for performing analysis of the CD4 + and CD8 + T lymphocyte count of the anticoagulated blood within 48 h. At room temperature,there were stable results for performing the analysis of CD4 + and CD8 + T lymphocyte count of processed samples within 24 h. Between 24 h and 48 h,although CD4 + count was stable,CD8 + count showed significant changes,so the ratio of CD4 to CD8 changed accordingly.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2004年第2期129-131,共3页
Chinese Journal of Experimental and Clinical Virology
