摘要
目的 观察不同浓度利多卡因对中枢海马锥体神经元N-甲基-D天冬氨酸(NMDA)介导钙电流的影响。方法 将已培养12-14 d的Wistar大鼠海马神经元按利多卡因浓度(10-5-10-1mol/L)分成5组,以不含利多卡因组做对照,共6组(n=6)。应用全细胞膜片钳方法,采用无间隙模式,采集记录各组大鼠海马神经元NMDA介导钙电流及各组静息电位的变化。结果 10-3、10-2、10-1组的电流密度较对照组降低(P<0.05或0.01);利多卡因浓度与电流密度呈直线负相关(r=0.76,P<0.01)。各组静息电位差异无显著性。结论 利多卡因可浓度依赖性抑制NMDA介导钙电流,低浓度利多卡因的神经保护作用可能与此有关。
Objective To investigate the effects of various concentrations of lidocaine on the N-methyl-D-aspartate ( NMDA)-mediated calcium currents in cultured rat hippocampal neurons. Methods Hippocampal neurons were obtained from newborn Wistar rats (0-24 h after birth) . The hippocampal neurons cultured for 12-14 days were divided into control group and 5 lidocaine groups in which lidocaine was added to the extracellular solution achieving the final concentrations of 10-3 , 10-4 , 10-3 , 10-2 and 10-1 . The neuronal cells were voltage clamped at - 80 mv. The currents evoked by NMDA 100 μmol·L-1 were recorded. The NMDA-mediated Ca currents were isolated by blocking Na-currents with TTX, K-currents with CsCL and TEACL and non-NMDA receptor (to a large degree AMPA receptor) with CNQX. Current density was calculated (pA / pF) .Results Lidocaine significantly reduced the density of NMDA-mediated calcium currents at concentrations of 10-3- 10-1 μmol·L-1 compared with that in the control group (P < 0.05 or 0.01) .Conclusion Lidocaine can inhibit calcium currents mediated by NMDA in a dose-dependent manner.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2004年第6期447-449,共3页
Chinese Journal of Anesthesiology
