摘要
以枯草芽孢杆菌T1 0 0 1为出发菌株 ,经紫外线、硫酸二乙酯逐级诱变处理 ,选育出腺嘌呤缺陷型 (Ade- )菌株 ,然后经蛋氨酸亚砜 (MSO)、8 氮鸟嘌呤 ( 8 AG)结构类似物平板定向筛选 ,获得鸟苷高产菌株TA2 0 8。通过模式识别法对发酵培养基的组成进行优化 ,同时对发酵条件如温度、pH值等进行了探索。在最优条件下 ,在 5L自控发酵罐上发酵 60h ,可产鸟苷 2 3 .68g/L。
Guanosine overproducing mutant was derived from Bacillus subtilis T1001 by using ultraviolet and diethyl sulfate (DES). The mutant was selected directionally and step wisely on the agar plates containing methionine sulfoxide(MSO) and 8 azaguanine(8 AG). The fermentation medium was optimized by pattern recognition method and the fermentation condition was also studied. Under the optimal condition, the mutant could produce guanosine 23.68 g/L after fermentation in 5L auto fermenter for 60 hours.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2004年第7期48-51,共4页
Food and Fermentation Industries
基金
天津市高等学校科技发展基金项目 (No .2 0 0 31 2 0 5 )
