摘要
本研究通过观察骨髓移植后小鼠骨髓中血小板内皮细胞间黏附分子 (PECAM 1/CD31)的表达水平变化及川芎嗪对其表达的调节作用 ,进一步探讨川芎嗪促进骨髓造血微环境修复、改善造血重建的作用机制。将BALB/c小鼠随机分为正常对照组 ,单纯BMT对照组和川芎嗪治疗组 (骨髓移植 +川芎嗪 ) ,接受 7.5Gy60 Coγ射线一次性全身均匀照射 ,照射后进行同基因小鼠骨髓移植 ,并分别胃饲等量生理盐水与川芎嗪注射液 ,2次 /天。骨髓移植后第 7,14 ,2 1天 ,采用流式细胞术检测小鼠骨髓有核细胞表面CD31分子表达水平 ,计数外周血白细胞、血小板及骨髓有核细胞数 ,并做骨髓组织学检查。结果表明 :骨髓移植后第 7,14 ,2 1天川芎嗪治疗组的CD31表达水平 ,外周血白细胞、血小板和骨髓有核细胞计数以及骨髓细胞增生程度均高于骨髓移植对照组 (P <0 .0 1或P<0 .0 5 )。结论 :川芎嗪可以明显促进骨髓移植后骨髓有核细胞表面黏附分子CD31的表达水平 ,这可能是其促进造血重建的作用机制之一。
To estimate the effect of ligustrazine on the expression of PECAM-1/CD31 and hematopoietic reconstitution in syngenic bone marrow transplantated mice,56 BALB/c mice were divided into 3 groups: normal control group,BMT control group,ligustrazine treated group (BMT+Ligustrazine) and syngenic BMT mouse models were established according to the literatures. The BMT control group and the ligustrazine treated group were given orally 0.2 ml saline and 2 mg ligustrazine twice a day respectively. On days 7,14,21 after BMT, mice were killed and peripheral blood cells,bone marrow nucleated cells (BMNC) were detected. Histological observation of bone marrow was made and the CD31 expression was assayed by flow cytometry. The results showed that in ligustrazine treated group the peripheral blood cell,BMNC counts on days 7,14,21 after BMT were higher than in the BMT control group (P<0.01 or P<0.05). The expression of CD31 in ligustrazine treated group on days 7,14,21 after BMT was also higher than in the BMT control group (P<0.01 or P<0.05). In conclusion,ligustrazine enhances CD31 expression in bone marrow cells after syngenic bone marrow transplantation of mice,which may be related to the mechanisms underlying the ligustrazine accelerating hematopoietic reconstitution in syngeneic bone marrow transplantation.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第4期489-493,共5页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目
编号 3 9870 92 6
