摘要
目的 探讨胎盘及脐血管中TGF - β1与同征发病机理的关系。方法 被研究人群由 2 0例正常产妇和 4 0例不同程度的妊高征患者组成 ,胎盘组织和脐带组织由剖宫产术后取得 ,用免疫组化ABC法染色观察TGF - β1在胎盘与脐带组织中的分布与定位 ,通过高清晰度彩色病理图文分析系统对其进行定量分析 ,并利用HE染色观察各组胎盘及脐血管的病理变化。结果 TGF - β1在各组胎盘合体滋养细胞层及脐动脉全层中均有不同程度的表达。中 ,重度妊高征组胎盘组织中 ,其TGF - β1的含量明显高于轻度妊高征组及正常组 (P <0 .0 5 ) ,正常组与妊高征组脐血管中TGF - β1的含量无显著性差异(P >0 .0 5 )。HE染色可见妊高征组胎盘合体细胞结节增多 ,纤维素样坏死 ,脐静脉内膜受损等病理改变。结论 妊高征患者胎盘组织中TGF - β1明显升高 ,提示TGF -
Objective: To investigate the relationship between the expression of transforming growth-β 1(TGF-β 1) in placenta and umbilical cord and the pathogenesis of pregnancy-induced hypertension(PIH). Methods: The study population consisted of 20 normal pregnancy women and 40 women PIH. Placental tissues and umbilical cords were obtained after caesarean. Immunohistochemistry was used toobserve the expression and distribution of TGF-β 1 in placenta tissues and umbilical cords. High resolution pathological image analysis system(HPIAS) was used to determine the quality of TGF-β 1. The pathology changes in placenta tissues and umbilical cords were observed by HE staining. Results: TGF-β 1 could express in syncytiotrophoblast and all layers ofumbilical vessels. The levels of TGF-β 1 expression of placenta on moderate and severe PIH patients were significant higher than that of normal group(P<0.05), and the expression of TGF-β 1 in umbilical cord was no significant difference between normal women and PIH patients (P>0.05). Nodules of syncytiotrophoblast, fibrinoid necrosis and damage of endometrium were. observed by HE staining. Conclusion: The levels of TGF-β 1 expression in PIH increases obviously and it may be involved in the pathogenesis of PIH.
出处
《中国优生与遗传杂志》
2004年第4期28-29,35,F003,共4页
Chinese Journal of Birth Health & Heredity
关键词
妊娠高血压综合征
胎盘
脐带
转化生长因子-Β1
Pregnancy-induced hypertension
Placenta
Umbilical cord, Transorming growth-β 1
