摘要
目的:探讨直接涂片ABC蛳AP染色在急性白血病免疫分型诊断中的价值。方法:对102例急性白血病患者的骨髓或外周血直接涂片,应用13种白细胞系列单克隆抗体,ABC免疫酶标法进行免疫学分型。结果:在73例ANLL中,纯髓系表型占90.4 %(66/73),粒单系特异性标记单抗表达率高低依次为CD+13 80.6%(58/72);CD+33 68.1 %(49/72);CD+15 55.6 %(40/72);29例ALL中,纯淋巴系表型为93.1%(27/29)。B细胞系特异性单抗标记表达率高低依次为CD+19 83.3 %(20/24);CD+22 70.8 %(17/24);CD+10 41.7 %(10/24)。T细胞系标记表达率高低依次为CD+5 和CD+7均为100 %(5/5);CD+2 80 %(4/5);CD+3 60 %(3/5)。102例急性白血病中,形态学分型正确归类者为96例,符合率为94.1 %(96/102)。结论:直接涂片ABC蛳AP染色,方法简便易行,结果可靠。
Objective: To explore the value of direct smear and ABC- AP staining method in immunophenotyping diagnosis of acute leukemias(AL). Methods: The immunophenotyping of 102 cases with AL were detected by 13 leukon monoclonal antibodies with direct smear of peripheral blood or bone marrow and ABC- AP staining method. Results: The result of imunophenotyping showed the pure myeloid phenotype was 90.4 % and the order of myeloid lineage-specific marker expressin was as follows CD+13 80.6 %(58/72);CD+33 68.1 %(49/72);CD+15 55.6 %(40/72);In 29 cases with ALL, The pure lymphocytic phenotype was 93.1 %(27/29), the order of lymphoid lineage- specific marker expression was as follows CD+19 83.3 %(20/24);CD+22 70.8 %(17/24);CD+10 41.7 %(10/24)in B- cell, and CD+5 and CD+7 100 %(5/5);CD+2 80 %(4/5);CD+3 60 %(3/5)in T- cell. 96 cases with AL were correctly classified by morphological classification in 102 cases with AL. The coincidence rate was 94.1 % with imunophenotyping. Conclusion: The direct smear and ABC- AP staining method are simple and convenient ,also its results are reliable. Imunophenotyping has important clinic value for the differential diagnosis of ALLand ANLL and the diagnosis of rare AL.
出处
《白血病．淋巴瘤》
CAS
2004年第4期209-211,共3页
Journal of Leukemia & Lymphoma
