摘要
目的 对海藻酸钠 聚左赖氨酸 海藻酸钠 (APA)微囊包裹杂交瘤细胞进行初步研究。方法 用人IgG1 免疫小鼠 ,取免疫后脾细胞与小鼠骨髓瘤细胞系SP2 0细胞融合 ,获得分泌抗人IgGκ链单克隆抗体 (mAb)的杂交瘤细胞株 ,命名为JY A1;通过优化制备条件 ,制备包裹杂交瘤细胞的APA微囊 ,并考察形态学 ;比较不同微囊膜的机械强度和化学强度 ;用ELISA法测定mAb的释放。小鼠腹腔注射微囊 ,不同时间回收观察。结果 相同条件下制得的微囊粒径均匀、表面光滑。体外培养条件下mAb能持续透过微囊膜。小鼠腹腔注射微囊后无异常 ,回收的微囊大部分形态完整。结论 采用高压静电成囊技术制备的APA微囊具有较高的膜强度 ,对细胞分泌产物mAb能持续稳定释放 。
Aim To study the cytomedicine of alginate-poly(L)lysine-alginate (APA) microencapsulated hybridoma cells and their characteristics. Methods The spleen cells taken from BALB/C mice immunized with purified human IgG 1κ type were fused with mouse myeloma cells SP2/0. The hybridoma cell lines secreting monoclonal antibodies (mAb) against human IgG 1κ type was named JY-A1.The APA microencapsulated JY-A1 cells were prepared with a high-voltage electrostatic system. Microencapsulation parameters were optimized and their morphology was studied. The mechanical strength and chemical intensity of microcapsules were measured. The mAb secrete from APA microencapsulated JY-A1 cells was determined by ELISA kit. The microcapsules injected into mice abdominal cavity previously were recovered at intervals. Results The microcapsules prepared in the same condition of the high-voltage electrostatic system were round and homogeneous. The mAb secreted by the microencapsulated JY-A1 cells were shown to permeate the membranes of APA microcapsules in vitro. After an intraperitoneal injection to mice, APA microcapsules were recovered on day 7, 14, 28, 56. The electron microscopy study revealed that the majority of recovered microcapsules were intact, and no evidence of immunological reaction in terms of fibrosis. Conclusion APA microencapsulated hybridoma cells prepared by high-voltage electrostatic system have good mechanical strength and chemical intensity. The APA microencapsulated hybridoma cells can maintain physiological functions in vitro, and the microcapsules have good biocompatibility in vivo.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第8期635-639,共5页
Acta Pharmaceutica Sinica
