摘要
背景:已有较多的研究证明脐血细胞能向神经元样细胞分化,且成功的运用脐血治疗脑卒中及其他神经系统疾病的研究已有报道,能否应用脐血干细胞治疗神经变性疾病尚未可知。目的:探讨应用人脐血间充质干细胞治疗大鼠帕金森病的可行性及其机制。设计:随机对照实验研究。地点和对象:健康Sprague-Dawley大鼠18只,清洁级,体质量220～260g。脐血标本取自郑州大学第三附属医院妇产科,每个标本60～120mL。干预:制备6-羟多巴胺帕金森病偏侧大鼠模型,随机分为3组:①对照组(n=6)。②PBS组(n=6):在每只大鼠右侧纹状体移植10μL的PBS。③间充质干细胞组(n=6):将3×106个用BrdU标记的脐血间充质干细胞植入大鼠右侧纹状体,4周后用阿朴吗啡诱发旋转试验并用免疫组织化学检测植入细胞的存活情况及纹状体酪氨酸羟化酶阳性细胞。主要观察指标:①移植前后各组大鼠旋转圈数。②免疫组织化学染色结果。结果:脐血间充质干细胞在体内存活,与对照组相比,间充质干细胞移植组阿朴吗啡诱发的旋转行为犤每30钟大鼠旋转圈数为212±60比340±30犦显著改善(P<0.05),但右侧纹状体酪氨酸羟化酶阳性细胞数与对照组相比无显著性差异(P>0.05)。结论:人脐血间充质干细胞脑内移植能改善帕金森大鼠的行为缺陷,可作为治疗神经变性疾病的一种潜在细胞?
BACKGROUND:Many researches have proved that cord blood cells can differentiate into neurons, and moreover,there are also reports regarding the successful application of cord blood in the treatments of cerebral apoplexy and other diseases of nervous system.However,it is still unknown whether cord blood stem cells can be used in the treatment of neurodegenerative diseases or not.OBJECTIVE:To investigate the feasibility and the mechanism of mesenchymal stem cells(MSCs) derived from human umbilical cord blood(HUCB) in the treatment of rats with Parkinson disease(PD).DESIGN:A randomized controlled trial.SETTING and MATERIALS:Eighteen healthy Sprague-Dawley (SD) rats of cleanness grade with a body mass from 220 g to 260 g were selected.Cord blood samples were obtained from the Department of Obstetrics and Gynecology of the Third Affiliated Hospital of Zhenzhou University.Each sample had 60 mL to 120 mL of cord blood.INTERVENTION:Lateral PD rat model induced by 6-hydroxydopamine was prepared.Rats were randomly divided into three groups:①control group(n=6).②PBS group (n=6):injection of 10 μL PBS into the right striatum.③MSCs group(n=6):injection of 3×106 MSCs(10 μL) marked with BradU into the right striatum, apomorphine induced rotational behavior was tested after 4 weeks of transplantation, and immunohistochemistry assay was carried out to trace the survival of MSCs and the tyrosine hydroxylase(TH)-immucoreactive cells in the striatum as well.MAIN OUTCOME MEASURES:①rotation rounds in rats of each group after transplantation.②the results of immunohistochemistry.RESULTS:MSCs survived in the striatum.The rotational behavior induced by apomorphine in rats of MSCs group[(212±60) rounds/30 minutes]was significantly improved compared with that of control group[(340±30) rounds/minutes](P< 0.05);However, the number of TH-positive cells in the right striatum had no statistical difference between MSCs and control group(P >0.05) . CONCLUSION:Intracerebral transplantation of mesenchymal stem cells derived human umbilical cord blood can alleviate neurological deficits in Parkinsonism rats,which may be a potential alternative cell source for the treatment of neurodegenerative disease.
出处
《中国临床康复》
CSCD
2004年第25期5460-5462,共3页
Chinese Journal of Clinical Rehabilitation
基金
河南省重点科技攻关基金资助项目(01170319)~~
