摘要
目的 建立人血浆中烟酸的高效液相色谱 (highperformanceliquidchromatography ,HPLC)测定方法。方法 以替硝唑为内标物 ,用乙腈直接沉淀人血浆中蛋白质 ,上清液在sphersorb色谱柱上 ,以乙腈 水 10 %四丁基氢氧化铵 (5 5∶4 5∶1)为流动相进行分离 ,流速 1ml·mim-1;柱温 30°C ;检测波长为 2 5 4nm。结果 烟酸与内标分离完全且无其他干扰 ,保留时间分别为 5 5 4、4 15min。线性范围 0 18～ 11 0 μg·ml-1,r=0 9999.日内RSD为 1 70～ 3 33% ,日间RSD为 1 75 %～ 4 11% ,回收率为 94 2 %～10 6 6 % ,血浆中烟酸的最低检测浓度为 0 0 9μg·ml-1。结论 所用方法简便、准确、重复性好 ,可用于口服烟酸缓释片后的血药浓度检测。
Objective TO establish a HPLC determination method for nicotinic acid in human plasma.Methods Using acetonitrile to deposit protein directly.The nicotinic acid and internal standard(Tinidazole) were separated on C 18 column with a mobile phase consisting of acetonitrile-water-10% tetrabutylammonium(55∶45∶1),with flow rate of 1ml·mim -1 ,and the detection wavelength was 254nm.Results Nicotinic acid was completely separated from the internal standard,and the retention time was 5.54min and 4.15min,respectively.The calibration curve was linear within 0.18～11.0μg·ml -1 ,r=0.999 9.The recoveries of methodology were 94.2%～106.6%.RSD within-day and between days were 1.70%～3.33% and 1.75%～4.11%,respectively.And the detection limit of nicotinic acid in human plasma was 0.09μg·ml -1 above.Conclusions The method is simple,accurate and repeatable.It has been successful in the determination of nicotinic acid in human plasma for pharmacokinetic studies.
出处
《广西医学》
CAS
2004年第9期1283-1285,共3页
Guangxi Medical Journal
关键词
烟酸
高效液相色谱
血药浓度
药物动力学
Nicotinic acid
HPLC
Drug concentration in plasma
Pharmacokinetics
