摘要
目的观察硫酸肝素-胶原蛋白支架材料与嗅鞘细胞的相容性。方法以冷冻干燥的方法制备硫酸肝素-胶原蛋白支架材料。培养、纯化并鉴定嗅鞘细胞后,将Hoechst荧光标记的嗅鞘细胞复合于硫酸肝素-胶原支架材料中,荧光显微镜、扫描电镜及光镜下观察其在材料内部的空间排列形式。以MTT法检测嗅鞘细胞与硫酸肝素-胶原蛋白共培养时的生物活性。结果以硫酸肝素复合胶原蛋白结合冷冻干燥技术制备的支架材料具有纵行的、平行排列的微管结构,嗅鞘细胞在支架材料微管内成线性平行排列,MTT法证明嗅鞘细胞与硫酸肝素-胶原蛋白材料共培养时保有高度的生物活性。结论硫酸肝素复合胶原蛋白结合特定条件下冷冻干燥技术可以制备在组分和内部空间结构上高度仿生的新型神经组织工程支架材料,其与嗅鞘细胞有良好的相容性。
Objective:To observe the compatibility of collagen-heparin sulfate scaffold with olfactory ensheathing cells.Method:Collagen-heparin sulfate scaffold was produced by freeze-drying.After the olfactory ensheathing cells were cultured,purified and indentified,they were Hoechst fluorescence labeled and compounded on collagen-heparin sulfate scaffold.The dimentional arrange of the cells were observed through fluorescence microscope,scanning electron microscope and light microscope.Bioactivity of olfactory ensheathing cells when co-culturing together with collagen-heparin sulfate scaffold was inspected by MTT method.Result:Collagen-heparin sulfate scaffold,produced by freeze-drying,had longitudinal and parallel microtubule structure.Olfactory ehsheathing cells were parallel arranged in linearity.MTT detection proved that olfactory enstheating cells preserved bioactivity while co-culturing with collagen-heparin sulfate scaffold.Conclusion:Collagen-heparin sulfate scaffold,produced under special freeze-drying condition,is a new kind of nervous tissue engineering scaffold,which is highly mimic natural nerve in space structure and component and has nice cellular compatibility with olfactory ensheathing cells.This new kind of scaffold has application prospect.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2004年第9期538-541,i002,共5页
Chinese Journal of Spine and Spinal Cord
基金
国家自然科学基金资助项目(30070761)
关键词
硫酸肝素
胶原
嗅鞘细胞
神经组织工程
细胞相容性
Heparan sulfate
Collagen
Olfactory ensheathing cell
Nervous tissue engineering
Cellular compatibiliaty
