摘要
本文运用一种模拟血流动力学状态的平板流动腔血管模型 ,研究在不同剪切力作用和不同处理因素对内皮细胞的粘附保留状态的影响。用不同因素处理细胞载片后在其上培养细胞 ,再用同一剪切力作用 ,或者用同一底物处理细胞载片后用不同剪切力作用。结果发现 ,在生理剪切力条件下 ,低剪切力 (2 .1Pa)作用时细胞保留率为 90 .38%± 2 .0 9% ,比高剪切力 (4 .2Pa)作用时细胞保留率 (4 6 .5 8%± 1.98% )要高 ;多聚赖氨酸处理时的细胞保留率 (90 .38%± 2 .0 9% )和纤维连接素处理时的细胞保留率 (91.5 9%± 1.2 4 % )比Ⅳ型胶原处理时细胞保留率 (19.77%± 1.0 5 % )要高 ,且不同底物对细胞脱落行为有不同影响 ;而血管内皮生长因子基因转染对细胞粘附无明显影响。此结果提示 ,低剪切力、多聚赖氨酸和纤维连接素有利于组织工程血管模型中的细胞粘附。
Aim To study the adhesion and retention of endothelial cells in different conditions, such as different shear stress and substrates, and gene trasfected or not, with the parallel plate flow chamber. Methods After treated with different substrates, endothelial cells were cultured on the substrates, and were processed by the same size shear stress. Or endothelial cells were processed by different size shear stress which cultured on the same substrate. Results Cells retention ratio was higher in low shear stress than high shear stress although both of them were in normal shear stress. Cells retention ratio was high in poly-l-lysine and fibronectin than in collagen Ⅳ. There was no effect on retention of endothelial cells in poly-l-lysine that gene transfected into endothelial cells. Conclusion Different size shear stress and substrates effect the retention of endothelial cells.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第4期399-401,共3页
Chinese Journal of Arteriosclerosis
基金
高等学校重点实验室访问学者基金
湖南省教委重点课题 ( 972 0 0 9)基金
