摘要
利用组织切片技术和电子显微镜研究江黄颡鱼[Pelteobagrusvachelli(Richardson)]精巢的精细胞结构。未成熟精巢中次级精母细胞的细胞核大,核物质染色浅;次级精母细胞的线粒体向细胞的一侧集中,有囊泡从细胞膜排出;精子细胞的核物质染色深,有明显的外排作用;精子分头部、中段和鞭毛三部分。头部长约1.8μm,有马鞍形的细胞核及不规则的核泡。中段有袖套、核小窝及中心粒复合体;核小窝非常发达,长度约为细胞核长轴的一半;中心粒复合体位于核小窝内。鞭毛长约15μm,其轴丝为典型的"9+2"结构。江黄颡鱼的精子不具顶体。
Ultrastructure of spermatozoa of darkbarbel catfish (Pelteobagrus vachelli) was investigated using histochemical methods and transmission and scanning electron microscopes. The spermatozoon consists of three partshead, midpiece and flagellum. The head (about 1.5μm in length) has a saddle-shaped nucleus with vesicles in it. The midpiece contains sleeve, nuclear fossa and centriolar complex. The centriolar complex is located in the nuclear fossa. The nuclear fossa, which is 1/2 of the head, is well developed. The flagellar tail is about 15μm in length containing the conventional '9+2' axoneme. The spermatozoa of P. vachelli are acrosome less.
出处
《上海水产大学学报》
CSCD
2004年第3期198-202,共5页
Journal of Shanghai Fisheries University
基金
安徽省科技厅2001年重点科研项目(0102308)
关键词
江黄颡鱼
精子
超微结构
Pelteobagrus vachelli
spermatozoa
ultrastructure
