摘要
目的:建立监测人血浆中咪达唑仑、三唑仑、艾司唑仑和阿普唑仑药物浓度的正相高效液相色谱方法。方法:血浆样品用乙醚萃取,55℃水浴中氮气吹干;残留物用乙醇溶解后进样。色谱柱为Shim-pack CLC-CN 柱(150 mm×6.0 mm,5μm),流动相为正己烷-无水乙醇-甲醇(76:18:6),流速为1.10 mL·min-1,柱温为:40℃,紫外检测波长为230 nm。结果:本方法可用于同时测定血浆中4种药物的浓度。咪达唑仑、三唑仑、艾司唑仑和阿普唑仑在0.050-20.0μg·mL-1范围内线性关系良好(r≥0.9996),准确度分别为98.9%,99.8%,101.8%及100.5%(n=6)。日内和日间RSD均不大于5.1%(n=6)。结论:本方法灵敏、特异性强、准确、简便易行,适用于咪达唑仑、三唑仑、艾司唑仑和阿普唑仑4种药物血浓度的临床监测。
Objective: To establish an NP - HPLC method for monitoring concentration of midazolam, estazolam, tri azolam and alprazolam in human plasma. Method: The plasma samples were extracted with aether. The organic phase was evaporated under nitrogen stream at 55 ℃in water bath. The remains were dissolved by absolute ethanol. Column: Shim - pack CLC - CN, 150 mm × 6. 0 mm ,5 μm; mobile phase: n - hexane - absolute ethanol - methanol (76: 18:6)at a flow rate of 1. 10 mL·?min-1. The column temperature was 40℃ ,UV detection wavelength was 230 nm. Results: The contents of four components could be determined simultaneously. The linear ranges of midazolam , estazolam, triazolam and alprazolam were 0. 050 - 20. 0 μg· ?mL -1 (r≥0. 9996). The accuracies were 98.9% , 99. 8% ,101. 8% ,100. 5% (n=6). Intra -day RSD and inter - day RSD were less than 5. 1% (n =6). Conclu tion: The method is sensitive, specific, accurate and convenient for monitoring concentration of midazolam, estazolam, triazolam and alprazolam in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第4期385-387,共3页
Chinese Journal of Pharmaceutical Analysis
