摘要
提出应用 HPLC-PAD-MS研究中药配伍多种化学成分的基本思路 ,指出应用色谱保留时间、紫外光谱及质谱等多信息、综合对照的方法确定合煎液中各峰的归属 ,从而进一步研究了中药的配伍规律 .首先建立了一套稳定、重复而可靠的液相色谱分析方法进行分离 ,再用统一的分析方法对分离的色谱图进行数据处理 .在 HPLC-PAD-MS分析中可得到各成分的保留时间、紫外光谱和分子量信息 .通过比较合煎液和单煎液中各成分的保留时间及分子量信息可确定合煎液各峰的归属 ,最后比较了单煎液和合煎液的化学成分变化 .结果表明 ,川芎和赤芍配伍后有一新峰产生 ,同时有两个成分含量发生变化 .
The theory of herb compatibility is essential part of traditional Chinese medicines(TCM) prescription. No work has been reported on changes of multiple components in TCM compatibility. It is the first time that basic idea about studying changes of multiple components in TCM compatility was put forward. High-performance liquid chromatography-photodiode array detector-mass spectrometry(HPLC-PAD-MS) was used in this study. Information of retention time, ultraviolet(UV) spectra and molecular weights was compared to confirm the origin of peaks in TCM co-decoction. Then, changes of multiple components in TCM compatibility were carried out. Uniform chromatographic method was the foundation of basic idea. Meanwhile, the origins of peaks in TCM co-decoction were breakthrough. To explore the chemical changes of compatibility of Radices paeoniae rubra and Litusticam wallichii, a reproducible and stable chromatographic method was established. Then, chromatographic data were processed with the uniform chromatographic method. Information of retention time, UV spectra and molecular weight was obtained in HPLC-PAD-MS run. By comparing retention time, UV spectra and molecular weight of each peak in co-decoction with those of Radices paeoniae rubra and Litusticam wallichii, the origin of each peak in co-decoction was confirmed. Finally, the chemical changes of compatibility of Radices paeoniae rubra and Litusticam wallichii in co-decoction were compared with those of combined-decoction. The results indicate that two peaks change in content and one peak regenerate after compatibility.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第9期1632-1636,共5页
Chemical Journal of Chinese Universities
基金
国家"九七三"计划项目 (批准号 :G19990 5 5 4)资助
关键词
液相色谱-质谱
中药
配伍
High-performance liquid chromatography-photodiode array detector-mass spectrometry(HPLC-PAD-MS)
Chinese medicine
Herb compatibility
