摘要
目的 观察不同剂量五氯酚钠 (Na -PCP)在不同处理后引发小鼠体内免疫细胞的DNA链断裂损伤和微核形成作用。方法 采用单细胞凝胶电泳法 (SCGE)分别测定ICR小鼠灌胃染毒 0 ,2 5 ,5 0和 10 0mg/kg后 3和 2 4h脾细胞、胸腺细胞和巨噬细胞的DNA链断裂损伤。同时观察了连续灌胃染毒 1个月 ,剂量分别为 0 ,6 2 5 ,12 5和2 5mg/kg时脾细胞DNA链断裂损伤情况 ;微核试验观察染毒 2 4和 4 8h骨髓嗜多染红细胞的微核形成。结果 在不同剂量五氯酚钠染毒 3h后 ,脾细胞出现DNA链断裂损伤的频率以及迁移距离在 5 0和 10 0mg/kg组明显高于对照 ;巨噬细胞在 10 0mg/kg时出现明显的DNA损伤 ;但胸腺细胞在研究剂量范围内未见明显变化 ;染毒 2 4和 4 8h后 ,所有观察细胞均未出现明显的DNA链断裂损伤。五氯酚钠处理 1个月未诱发脾细胞明显的DNA链断裂损伤。骨髓嗜多染红细胞微核在研究剂量范围内未明显增高。结论 10 0mg/kgNa-PCP可诱发小鼠体内脾脏细胞和巨噬细胞的DNA链断裂损伤 ,5 0mg/kgNa -PCP可诱导脾脏细胞DNA链断裂损伤 ,Na -PCP对胸腺细胞DNA损伤作用不明显 :Na-PCP连续 1个月作用未诱导脾细胞出现明显的DNA链断裂损伤。 2 5～ 10 0mg/kgNa-PCP未诱导骨髓嗜多染红细胞微核增加。
Objective To investigate DNA strand breaks and micronuclei induced by sodium pentachlorophen (Na-PCP) in different phases and different dosages in vivo.Methods Mice were treated through gavage with Na-PCP at a dose of 0,25,50 and 100、mg/kg once time and 0,6.25,12.5 and 25、mg/kg for one month.Mice were sacrificed at the end of 3,24 and 48 hours after once treatment or after one month treatment.DNA strand breaks were detected in the splenocyte,thymocyte,and macrophage through SCGE(Single Cell Gel Electrophoresis).At the same time after once treatment micronuclei in polychromatic erythrocyte in bone marrow were also detected.Results It was showed that 3-hours different dosages treatment of 50 and 100、mg/kg Na-PCP could induce DNA damage not only in the frequency of comet cell but also in the migration of DNA breaks in cells from spleen.Only 100、mg/kg Na-PCP could induce DNA damage in macrophage.In contrast there was no change in cells from thymus in different dosage treatment and different treating period.Doring the research of different time-course study it was presented that 24-hour and 48-hour treatment was with no significant DNA strand breaks in each kind of cells.Na-PCP did not induce micronuclei in polychromatic erythrocyte under the condition of this assay.Conclusion 50 and 100、mg/kg Na-PCP could induce DNA strand breaks in cells from spleen.100、mg/kg Na-PCP could induce DNA strand breaks in macrophage.Thymus was insensitive to this kind of damage induced by Na-PCP and Na-PCP did not induce the increase of micronuclei in polychromatic erythrocyte under the condition of this assay.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第8期950-952,共3页
Chinese Journal of Public Health
关键词
五氯酚钠
免疫细胞
DNA链断裂
微核
sodium pentachlorophen
immunocyte
DNA strand breaks
micronuclei
