摘要
目的 探讨灯盏花素对大鼠糖尿病模型肾组织转化生长因子 β1(TGF β1)与结缔组织生长因子 (CTGF)表达的影响。方法 建立STZ诱导的单侧肾切除糖尿病模型 ,随机分 :对照组、模型组、灯盏花素给药组 (2 0mg·kg-1·d-1,灌胃 )。观察 8wk后大鼠体重、肾重、肾重 /体重、2 4h尿白蛋白排泄率 (AER)、肾小球面积 (AG)和容量 (VG)及系膜区面积 (AM)的变化 ,并检测肾组织与尿MDA含量及肾组织SOD、CAT、GSH PX活性 ,通过免疫组化检测肾小球TGF β1与CTGF蛋白表达。结果 灯盏花素给药对大鼠糖尿病模型血糖、体重无明显影响 ,可明显抑制肾重、肾重 /体重、AER、AG、VG 及AM 的增加 (P <0 0 5 ) ;对肾组织及尿MDA含量的增加有明显抑制作用 (P <0 0 5 ) ,并明显提高肾组织SOD、CAT及GSH PX活性 (P <0 0 5 ,P <0 0 1)。免疫组化半定量分析显示 ,与对照组相比糖尿病大鼠肾小球TGF β1及CTGF表达明显增加 (P <0 0 1) ,灯盏花素给药对其有明显抑制作用 (P <0 0 5 )。结论 灯盏花素对大鼠糖尿病模型肾脏有明显保护作用 ,其机制部分与抑制肾组织TGF
Aim To investigate the effect of breviscapine on the expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF)in kidney in diabetic rats. Methods Rats were randomly separated into three groups: control group, diabetic group, diabetic group treated with breviscapine ( 20 mg·kg -1·d -1 by gavage). Body weight(BW), kidney weight (KW), ratio of KW to BW (KW/BW) and 24 hours albumin excretion rate (AER) as well as glomerular area (A G), glomerular volume(V G), mesangial area (A M) were measured at week 8. The levels of MDA in renal tissue and urine as well as activities of superoxide diamutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) in renal tissue were determined. Immunohistochemistry for TGF-β1 and CTGF were performed in glomeruli.Resluts Increased KW, KW/BW, AER and A G, V G, A M were significantly attenuated by treatment with breviscapine (P<0.05). Elevated MDA levels as well as decreased SOD, CAT, GSH-PX activities in renal tissue were significantly remitted by breviscapine(P<0.05, P<0.01, respectively). Compared with those in control group, glomerular expression of TGF-β1 and CTGF were significantly increased in diabetic rats, which were all significantly inhibited by breviscapine(P<0.05). Conclusion The mechanism of renoprotection of breviscapine may be at least partly correlated with supression overexpression of TGF-β1 and CTGF in diabetic kidney.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2004年第9期1050-1054,共5页
Chinese Pharmacological Bulletin
基金
安徽省自然科学基金资助项目
No 0 10 43 70 3
安徽省教育厅资金资助项目
No 2 0 0 3kj173
